Anti-Mevalonate Kinase (MVK) Rabbit Monoclonal Antibody for WB, ELISA - Q03426

Core Product Specifications and Parameters

Biological Background: Mevalonate Kinase Function and Localization

• Mevalonate kinase (MVK) catalyzes the phosphorylation of mevalonate to mevalonate 5-phosphate, a key step in isoprenoid and cholesterol biosynthesis. Related references: PMID:11278915 PMID:18302342 PMID:9325256
• The enzyme localizes to the cytoplasm and peroxisomes.
• MVK is an ATP- and magnesium-dependent transferase/kinase.
• It is a central enzyme in lipid biosynthesis and sterol metabolism.
• According to UniProt, MVK is linked to disease variants and cataract.

Experimental Guidance and Technical Tips

• The immunogen resides within the C-terminal region (aa 297–396) of human MVK, a domain likely accessible in both native and denaturing conditions, supporting utility in WB and ELISA.
• For WB, consider using whole-cell or tissue lysates known to express MVK (e.g., liver, kidney). Validate antibody specificity with positive and negative controls.
• In ELISA, pairing this antibody with a matched capture/detection antibody may improve assay sensitivity. Perform titration experiments to optimize working concentrations.
• Cross-reactivity with other species has not been extensively characterized; for non-human samples, confirm specificity through sequence alignment and experimental validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with reliable access to high-quality IVD raw materials, including this anti-MVK monoclonal antibody. Our portfolio encompasses validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets within the cholesterol biosynthesis and metabolic disease research area. Partner with us to secure a seamless supply chain from concept to clinical application.