Anti-METTL9 Rabbit Polyclonal Antibody for WB, ELISA - Q9H1A3

Core Product Specifications and Parameters

Biological Background: METTL9 Function and Localization

• METTL9 is a protein-L-histidine N-pros-methyltransferase that catalyzes 1-methylhistidine (pros-methylhistidine) modification on target proteins. Related references: PMID:33563959, PMID:34562450, PMID:37015930
• It specifically recognizes a His-x-His (HxH) motif, methylating the second histidine residue while the first histidine serves as a recognition signature. Related references: PMID:37398635
• Known substrates include S100A9, NDUFB3, SLC39A5, SLC39A7, ARMC6, and DNAJB12; 1-methylhistidine modification may influence metal ion binding. Related references: PMID:33563959, PMID:34562450, PMID:37015930
• METTL9 is the primary cellular methyltransferase responsible for the 1-methylhistidine mark. Related references: PMID:33563959
• Subcellularly, METTL9 localizes to the endoplasmic reticulum and mitochondria.
• Alternative splicing produces multiple isoforms, and the protein is predicted to contain a signal peptide and N-linked glycosylation sites.
• Experimental three-dimensional structures have been resolved for METTL9.
• The predicted molecular weight is approximately 36.5 kDa.

Experimental Guidance and Technical Tips

• The antibody was generated using recombinant full-length human METTL9 protein as immunogen. It may detect multiple isoforms or post-translationally modified forms; validate the band pattern in your system.
• For western blotting, consider using total cell lysates from human or mouse cell lines known to express METTL9 as positive controls. The predicted molecular weight is ~37 kDa, but observe actual migration based on sample conditions.
• In ELISA applications, the recombinant immunogen can serve as a positive coating antigen and standard for quantification.
• Given cross-reactivity with mouse, this antibody is suitable for studies in mouse models; however, perform independent validation for mouse sample types.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials, including validated antibodies and ancillary reagents, to accelerate your diagnostic and research workflows. This anti-METTL9 polyclonal antibody supports investigations into protein histidine methylation and its role in metal homeostasis and signaling. As your one-stop partner, CamelBio also offers bulk custom antibody production, rare target sourcing, and technical consulting to meet your project needs from discovery to clinical application.