Anti-METTL8 Rabbit Polyclonal Antibody for WB, ELISA - Q9H825

Core Product Specifications and Parameters

Biological Background: METTL8 Function and Localization

• METTL8 (methyltransferase-like protein 8) is also known as tRNA N(3)-cytidine methyltransferase METTL8, mitochondrial, and mRNA N(3)-methylcytidine methyltransferase METTL8. It belongs to the methyltransferase superfamily and utilizes S-adenosyl-L-methionine (SAM) as a methyl donor.
• As a mitochondrial enzyme, METTL8 specifically catalyzes N(3)-methylcytidine (m3C) modification at position 32 of the anticodon loop of mitochondrial tRNA(Ser)(UCN) and tRNA(Thr). This modification is crucial for mitochondrial translation efficiency and proper respiratory chain function. Related references: PMID:34774131, PMID:35017528
• The methylation activity of METTL8 on mitochondrial tRNA(Ser)(UCN) requires prior N(6)-dimethylallyladenosine(37) (i6A37) modification by the enzyme TRIT1, indicating a hierarchical modification pathway. Related references: PMID:34774131, PMID:35017528
• METTL8 has been reported to mediate m3C modification on certain mRNAs, but the prevalence and biological significance of this activity in vivo remain uncertain and require further validation. Related references: PMID:28655767, PMID:33313824
• Subcellularly, METTL8 localizes predominantly to mitochondria, consistent with its role in mitochondrial tRNA modification. The protein possesses a transit peptide for mitochondrial import.
• The METTL8 gene undergoes alternative splicing, potentially yielding multiple isoforms. UniProt lists the reviewed isoform with a molecular weight of approximately 33.4 kDa.
• Key protein features include isopeptide bond formation, Ubl conjugation, and classification under tRNA processing and mitochondrial keywords.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody is a recombinant protein covering the N-terminal region (amino acids 1-140) of human METTL8. This region may include parts of the transit peptide and early catalytic domain.
• For Western blot (WB) applications, consider using mitochondria-enriched lysates or whole-cell extracts from human, mouse, or rat samples. The predicted molecular weight is ~33 kDa, but post-translational modifications or isoform variations may shift the band.
• In ELISA, this polyclonal antibody may detect native and denatured METTL8. Validate the signal specificity using appropriate controls, such as METTL8 knockdown or knockout cell lines.
• Cross-reactivity with mouse and rat METTL8 has been indicated; however, sequence homology should be checked, and optimal working conditions should be determined empirically in your experimental system.
• For functional studies linking METTL8 to mitochondrial translation or respiratory chain activity, consider using this antibody in conjunction with mitochondrial isolation and activity assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with high-quality IVD raw materials, including this anti-METTL8 antibody for mitochondrial biology and epitranscriptomic research. Beyond individual antibodies, we offer validated antibody pairs, optimized monoclonal and polyclonal preparations, bulk ancillary reagents, and rare-target material sourcing to accelerate your assay development. Partner with CamelBio for a streamlined procurement experience from concept to clinic.