Anti-METTL7B Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q6UX53

Core Product Specifications and Parameters

Biological Background: METTL7B Function and Localization

• METTL7B, also known as TMT1B (Thiol S-methyltransferase TMT1B) or methyltransferase-like protein 7B, is a thiol S-methyltransferase enzyme that catalyzes methyl group transfer from S-adenosyl-L-methionine (SAM) to thiol-containing substrates.
• It primarily functions in the endoplasmic reticulum of hepatocytes, accounting for the majority of S-thiol methylation activity and selectively methylating S-centered nucleophiles such as hydrogen sulfide and dithiothreitol.
• Subcellular localization includes the endoplasmic reticulum membrane, lipid droplets, microsomes, and cytosol, reflecting its involvement in membrane-associated metabolic processes.
• The protein is predominantly expressed in the liver, consistent with its hepatic thiol metabolism role, and has a calculated molecular weight of approximately 27.8 kDa (UniProt).
• As a member of the methyltransferase superfamily, METTL7B contains a conserved SAM-dependent methyltransferase domain and a signal peptide for targeting to cellular membranes.
• Its functions are critical for maintaining cellular thiol homeostasis and may influence lipid metabolism at the interface of the endoplasmic reticulum and lipid droplets.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 165–244 of human METTL7B, a region likely within the catalytic methyltransferase domain; consider this when assessing potential cross-reactivity or blocking peptide controls.
• For Western blot, a band around 28 kDa is expected; validate specificity using liver-derived cell lines (e.g., HepG2) as positive controls and knockdown/knockout samples.
• In immunofluorescence or ICC, anticipate a staining pattern consistent with endoplasmic reticulum and lipid droplet localization; use organelle markers for co-localization verification.
• The antibody is polyclonal and may exhibit lot-to-lot variability; optimize dilution and incubation conditions for each new batch and application.
• ELISA applications may benefit from employing the recombinant protein immunogen as a standard to quantify METTL7B levels in biological fluids or lysates.
• Since METTL7B expression is liver-specific, verify endogenous levels in non-hepatic cells to establish baseline staining or background for your assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering high-quality antibodies like this anti-METTL7B pAb together with validated antibody pairs, bulk reagents, and ancillary supplies. Whether your focus is liver metabolism, methyltransferase research, or diagnostic development, we provide one-stop access to optimized monoclonal/polyclonal antibodies and technical support. Our team can also assist in sourcing rare targets and custom raw materials to accelerate your project pipeline.