Anti-METTL7A Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9H8H3

Core Product Specifications and Parameters

Biological Background: METTL7A Function and Localization

• METTL7A (Methyltransferase-like protein 7A) is a thiol S-methyltransferase that catalyzes methyl transfer from S-adenosyl-L-methionine to alkyl and phenolic thiol-containing substrates, accounting for most S-thiol methylation activity in the hepatic endoplasmic reticulum together with TMT1B. Related references: PMID:37137720
• The protein localizes to the endoplasmic reticulum, from which it is targeted to lipid droplets, and is additionally found in microsomes and the cytosol; it plays a structural role in recruiting cellular proteins to lipid droplets to form functional organelles. Related references: PMID:19773358
• METTL7A functions as an N6-adenosine-methyltransferase for long non-coding RNAs (lncRNAs), facilitating the loading of methylated lncRNAs into exosomes at the tumor-stroma interface. Related references: PMID:34980213
• It promotes osteogenic and odontogenic differentiation by regulating the expression of genes critical for stem cell differentiation and survival. Related references: PMID:34226523, PMID:34790668
• Exhibits tissue-specific expression, with prominent levels in the liver.
• May be involved in the assembly and release stages of the hepatitis C virus life cycle, suggesting a role in host-virus interaction.
• Classified under keywords including Methylation, Methyltransferase, Transferase, and Host-virus interaction, and contains a signal peptide.

Experimental Guidance and Technical Tips

• For Western blotting, a band near 28 kDa is expected in lysates from liver or other METTL7A-expressing tissues; validate using appropriate positive and negative controls.
• IHC-P analysis may show positive staining in hepatocytes; consider using liver sections as a positive control and verify with isotype controls.
• In IF/ICC, anticipate labeling of the endoplasmic reticulum and lipid droplets; co-staining with organelle markers can help confirm localization.
• ELISA can be developed using recombinant METTL7A protein as a coating antigen; optimize coating concentration and detection conditions for your assay format.
• Due to the polyclonal nature of this antibody, assess lot-to-lot consistency if used in quantitative or regulated IVD applications.
• Cross-reactivity with mouse and rat is expected based on sequence homology, but confirm reactivity in your specific model system before large-scale studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with integrated access to IVD raw materials, technical services, and consulting—from concept to clinic. This METTL7A polyclonal antibody supports research into methylation biology, liver function, and viral host interactions, and is backed by our supply chain expertise for bulk antibody pairs, ancillary reagents, and custom sourcing of related rare targets. Whether scaling production or initiating novel diagnostic development, CamelBio streamlines your raw-material procurement.