Anti-METTL4 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q8N3J2

Core Product Specifications and Parameters

Biological Background: METTL4 Function and Localization

• METTL4 (methyltransferase-like protein 4) is an N(6)-adenine-specific methyltransferase capable of modifying both RNA and DNA molecules. Related references: PMID:31913360, PMID:32183942

• It catalyzes formation of N6,2'-O-dimethyladenosine (m6A(m)) on internal positions of U2 small nuclear RNA (snRNA), a modification that regulates pre-mRNA splicing. Related references: PMID:31913360

• METTL4 also functions as a DNA N(6)-adenine methyltransferase, although the biological role of m6A on mammalian DNA remains under investigation and requires further confirmation. Related references: PMID:32183942, PMID:32203414

• In the mitochondrial matrix, METTL4 methylates mitochondrial DNA (mtDNA), repressing transcription by inhibiting TFAM binding and DNA bending, thereby influencing mtDNA copy number and mitochondrial gene expression. Related references: PMID:32183942

• The enzyme contributes to Polycomb silencing by promoting ubiquitination and degradation of ASXL1 and MPND, leading to inactivation of the PR-DUB complex (By similarity).

• Subcellularly, METTL4 localizes to the nucleus, cytosol, and mitochondrial matrix, reflecting its involvement in both nuclear and mitochondrial nucleic acid methylation.

• As a member of the methyltransferase family, it uses S-adenosyl-L-methionine as a methyl donor and is implicated in chromatin regulation.

Experimental Guidance and Technical Tips

• Immunogen design: The antibody was raised against a recombinant protein encompassing the N-terminal region (aa 1–190) of human METTL4, a segment that shows conservation across human, mouse, and rat, supporting cross-reactivity.
• Western blotting: In WB, a band near 54 kDa is anticipated; use mitochondrial-enriched or whole-cell lysates and titrate the antibody for optimal signal-to-noise.
• IHC-P / IF-ICC: For staining, we suggest antigen retrieval and permeabilization steps, and validation in METTL4-expressing tissues or cell lines.
• ELISA: This antibody can be employed in sandwich or direct ELISA formats; include appropriate recombinant protein standards for quantification.
• Cross-reactivity: While reactivity with human, mouse, and rat is reported, end-users should verify performance in their specific sample type and species.

CamelBio: Your One-Stop Sourcing Bridge

Targeting epigenetic regulators such as METTL4 demands reliable antibody reagents. CamelBio serves diagnostic manufacturers and research laboratories by offering validated polyclonal and monoclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets. As your one-stop IVD raw-material partner, CamelBio streamlines the supply chain from concept to clinical application.