Anti-METTL21C Rabbit Polyclonal Antibody for WB, ELISA - Q5VZV1

Core Product Specifications and Parameters

Biological Background: METTL21C Function and Localization

• METTL21C, also referred to as Methyltransferase-like protein 21C, is a protein-lysine N-methyltransferase that utilizes S-adenosyl-L-methionine (SAM) as a methyl donor. Related references: PMID:22948820, PMID:32611769
• It specifically catalyzes mono-, di-, and trimethylation of lysine 943 on alanyl-tRNA synthetase (AARS1), potentially modulating AARS1 function or interactions. Related references: PMID:32611769
• The protein is localized to both the nucleus and cytoplasm, indicating involvement in diverse cellular processes.
• METTL21C belongs to the methyltransferase family, with structural studies revealing its 3D configuration.
• The full-length protein has a predicted molecular weight of approximately 29.6 kDa; the antibody detects a band at 30 kDa, consistent with immunoassay data.
• Keywords associated with METTL21C include methyltransferase, transferase, cytoplasm, nucleus, and S-adenosyl-L-methionine, highlighting its catalytic and localization properties.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein corresponding to amino acids 1-264 of human METTL21C (NP_001010977.1), covering the full-length sequence; this polyclonal antibody may recognize multiple epitopes.
• For Western blotting, a band around 30 kDa is expected; consider using whole-cell lysates or relevant tissue extracts and validate with appropriate positive and negative controls.
• The antibody cross-reacts with mouse and rat METTL21C, expanding its utility for comparative or translational studies.
• ELISA applications may require standard titration to determine optimal coating concentrations and detection ranges; validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, including validated antibody pairs, recombinant proteins, and ancillary reagents. For methylation research and gene regulation studies, we offer polyclonal and monoclonal antibodies like this anti-METTL21C antibody, backed by technical support from concept to clinic. Our extensive supply network ensures reliable sourcing of rare targets and bulk raw materials to accelerate your assay development.