Anti-METTL14 Monoclonal Antibody (KD Validated) for WB, IHC-P, IP, ELISA - Q9HCE5

Core Product Specifications and Parameters

Biological Background: METTL14 Function and Localization

• METTL14 (Methyltransferase-like protein 14, also known as hMETTL14) is the non-catalytic subunit of the N6-adenosine-methyltransferase complex.
• In the heterodimer with METTL3, METTL14 serves as the RNA-binding scaffold that recognizes substrate mRNA, while METTL3 provides the catalytic activity (PubMed:27281194, PubMed:27373337, PubMed:27627798). Related references: PMID:27281194, PMID:27373337, PMID:27627798
• The complex catalyzes N6-methyladenosine (m6A) modification on adenine residues within the consensus motif 5'-[AG]GAC-3', regulating mRNA stability and processing (PubMed:24316715, PubMed:24407421, PubMed:25719671). Related references: PMID:24316715, PMID:24407421, PMID:25719671
• m6A methylation functions as a key regulator of mRNA stability by promoting transcript destabilization and degradation, affecting gene expression dynamics.
• METTL14-mediated m6A is critical for circadian clock regulation, embryonic stem cell differentiation, and cortical neurogenesis (PubMed:24316715, PubMed:24407421, PubMed:25719671). Related references: PMID:24316715, PMID:24407421, PMID:25719671
• The protein localizes predominantly to the nucleus.
• METTL14 is also involved in spermatogenesis and male fertility, regulating spermatogonial differentiation and meiosis (By similarity).
• METTL14 is a phosphoprotein identified in proteomic analyses, with a predicted molecular weight of 52,150 Da.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein spanning amino acids 1-210 of human METTL14 (NP_066012.1). Since this region is at the N-terminus, consider its conservation across species if cross-reactivity is desired; sequence alignment can assist in predicting reactivity in additional species.
• For Western blot (WB), a single band at approximately 52 kDa is expected. Validate detection in the appropriate species tissue/cell lysates, including human, mouse, and rat.
• In immunohistochemistry (IHC-P), METTL14 shows nuclear staining. Optimize antigen retrieval conditions and consider using known positive tissues (e.g., brain, testis) as controls.
• For immunoprecipitation (IP), this rabbit monoclonal antibody can efficiently capture METTL14 complexes; include appropriate isotype controls and confirm co-immunoprecipitation of METTL3 if studying the methyltransferase heterodimer.
• ELISA: The antibody can be used as a capture or detection antibody if paired with a compatible secondary for METTL14 quantification; validate the assay dynamic range and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers this anti-METTL14 monoclonal antibody along with a comprehensive range of IVD raw materials, including validated antibody pairs, optimized recombinant proteins, and ancillary reagents for m6A methylation research. Our one-stop sourcing platform supports diagnostic manufacturers from concept to clinic, ensuring rapid access to high-quality tools for epigenetic and epitranscriptomic assay development. Whether you need bulk quantities, custom formulations, or rare target-specific reagents, CamelBio bridges the gap between raw material innovation and your IVD product realization.