Anti-METTL13 Polyclonal Antibody for WB, ELISA - Q8N6R0

Core Product Specifications and Parameters

Biological Background: METTL13 Function and Localization

• METTL13, also known as eEF1A lysine and N-terminal methyltransferase (EEF1AKNMT), is encoded by the gene with aliases FEAT, KIAA0859, and others.
• Functions as a dual methyltransferase that catalyzes methylation of elongation factor 1-alpha (EEF1A1 and EEF1A2) at two distinct positions, playing a role in the regulation of mRNA translation.
  Related references:
  PMID:30143613
  PMID:30612740
• The C-terminal domain mediates N-terminal methylation of EEF1A at Gly-2, while the N-terminal domain dimethylates EEF1A at Lys-55.
  Related references:
  PMID:30143613
  PMID:30612740
• Exhibits specificity for EEF1A and does not methylate core histones H2A, H2B, H3, and H4.
  Related references:
  PMID:30612740
• Localizes to the cytoplasm, nucleus, and mitochondria, as indicated by subcellular localization annotations.
• Post-translationally modified by acetylation and phosphorylation; alternative splicing yields multiple isoforms.
• Note: A 3D structure is available for this protein, and it has been identified in high-throughput proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering amino acids 540–699 of human METTL13, which corresponds to the C-terminal domain. This region is responsible for the N-terminal methylation of EEF1A at Gly-2. Consider epitope accessibility and potential cross-reactivity when designing detection strategies.
• For Western blot (WB), a protein with a molecular weight of approximately 79 kDa is expected. Validate detection in relevant human, mouse, or rat samples using appropriate positive controls.
• In ELISA applications, consider using recombinantly expressed METTL13 protein or synthetic peptide to optimize antibody titers and ensure specificity. Include negative controls to confirm minimal cross-reactivity.
• Because METTL13 localizes to the cytoplasm, nucleus, and mitochondria, consider subcellular fractionation to enrich for the target protein when detection is challenging in whole-cell lysates.
• As METTL13 undergoes alternative splicing, be mindful that multiple isoforms may be detected. Verify the expected band pattern with appropriate molecular weight markers and isoform-specific references.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive sourcing solution for IVD raw materials, providing manufacturers and researchers with this METTL13 polyclonal antibody and a range of ancillary reagents for protein methylation and translational research. From optimized antibody pairs to bulk reagent supply, CamelBio streamlines your procurement and supports your development pipeline from concept to clinic.