Anti-Methylmalonyl-CoA Mutase Rabbit Monoclonal Antibody for WB - P22033

Core Product Specifications and Parameters

Biological Background: Methylmalonyl-CoA Mutase Function and Localization

• Methylmalonyl-CoA mutase (MUT, also known as MCM or MUTA) is a mitochondrial enzyme that catalyzes the reversible isomerization of methylmalonyl-CoA to succinyl-CoA, a key intermediate of the tricarboxylic acid cycle.
• This reaction is essential for the metabolism of branched-chain amino acids, odd-chain fatty acids, and cholesterol, linking degradation pathways to energy production.
• The enzyme is annotated with the keyword “Cobalamin,” indicating it requires adenosylcobalamin (coenzyme B12) as a cofactor, with cobalt ion coordination critical for its catalytic activity.
• Subcellular localization is primarily in the mitochondrial matrix, though it may also be detected in the cytoplasm.
• Mutations in the MMUT gene cause methylmalonic aciduria, an inborn error of metabolism, underscoring its clinical significance.
• The protein undergoes post-translational modifications such as acetylation and phosphorylation, which may regulate its activity or stability.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a peptide within amino acids 500–600 of human MUTA. Consider this epitope location when designing experiments or pairing with other antibodies.
• For Western blotting, the predicted molecular weight is approximately 83 kDa; validate the observed band in your specific sample system.
• In immunohistochemistry and immunofluorescence, appropriate antigen retrieval and fixation methods may enhance signal detection; optimization is recommended.
• This antibody can be used in ELISA for detection or capture applications; assay conditions should be empirically determined.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive portfolio of high-quality IVD raw materials, including validated monoclonal and polyclonal antibodies like this MUTA clone. Beyond antibodies, we offer bulk ancillary reagents, optimized antibody pairs, and expert support for rare target sourcing, helping accelerate your metabolic disease and enzyme research programs.