Anti-METAP2 Rabbit Polyclonal Antibody for WB and ELISA - P50579

Core Product Specifications and Parameters

Biological Background: Methionine aminopeptidase 2 Function and Localization

• METAP2 (methionine aminopeptidase 2), also known as p67eIF2, cotranslationally removes the N-terminal methionine from nascent proteins when the second residue is small and uncharged (e.g., Ala, Cys, Gly, Pro, Ser, Thr, Val). Its catalytic activity is particularly high toward Met-Val peptides.
• It protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by kinases like PKR and HCR, playing a critical role in regulating protein synthesis.
• Classified as a metalloprotease (peptidase M24 family), METAP2 requires metal cofactors for its aminopeptidase activity and is subject to acetylation, phosphorylation, and glycosylation.
• Originally identified as a 67 kDa glycoprotein (p67), the calculated molecular mass is 52.9 kDa; the protein weight on SDS-PAGE is approximately 53 kDa.
• Predominantly cytoplasmic, METAP2 associates with the ribosome to process nascent polypeptides.
• Alternative splicing of the METAP2 gene generates multiple transcript variants.
• Keywords: 3D-structure, acetylation, alternative splicing, aminopeptidase, cytoplasm, glycoprotein, hydrolase, metal-binding, phosphoprotein, protease.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal amino acids 1–220; thus, the antibody may detect isoforms containing this region. Confirm specificity in relevant samples.
• For Western blotting, expect a band around 53 kDa; post-translational modifications (e.g., glycosylation) may cause migration shifts. Use appropriate molecular weight markers.
• In ELISA, perform a titration to determine the optimal antibody concentration for coating or detection, depending on the assay format.
• Validate the antibody in positive control lysates (e.g., HeLa, HEK293) where METAP2 is ubiquitously expressed. Mouse cross-reactivity suggests compatibility with mouse models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with a comprehensive portfolio of IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies like this anti-METAP2 product. Whether you need bulk ancillary reagents or rare target sourcing, our one-stop platform covers everything from concept to clinic. For protein synthesis and translational control research, we offer reliable raw materials to accelerate your assay development.