Anti-MET Rabbit Polyclonal Antibody for WB and ELISA - P08581

Core Product Specifications and Parameters

Biological Background: Hepatocyte Growth Factor Receptor (MET) Function and Localization

• MET (c-Met, HGFR) is a transmembrane receptor tyrosine kinase that specifically binds hepatocyte growth factor (HGF), triggering pleiotropic cellular responses including proliferation, motility (scattering), morphogenesis, and survival.
• Upon HGF-induced dimerization, MET autophosphorylates cytoplasmic tyrosine residues, creating docking sites for adaptor proteins such as GRB2, GAB1, and SRC, which in turn activate the RAS/ERK, PI3K/AKT, and PLCγ/PKC signaling cascades to drive cytoskeletal reorganization and transcriptional programs.
• MET signaling is essential during embryogenesis (gastrulation, myoblast migration, liver and kidney development) and in postnatal processes such as wound healing, organ regeneration, and bone homeostasis.
• The mature MET receptor is a heterodimer consisting of an extracellular α chain and a single-pass transmembrane β chain harboring the tyrosine kinase domain; it is localized primarily at the plasma membrane, and a soluble form can be generated through ectodomain shedding.
• In humans, MET is widely expressed in epithelial cells of the liver, gastrointestinal tract, thyroid, kidney, and skin, with particularly high levels in hepatocytes, gastric and intestinal epithelium, and basal keratinocytes; it is also found in the brain and in metaphyseal bone at the protein level.
• As an entry receptor for Listeria monocytogenes via its interaction with internalin InlB, MET illustrates a pathophysiological mechanism of microbial invasion, and its aberrant activation (via mutations, gene amplification, or overexpression) is implicated in oncogenic transformation, making it a prominent therapeutic target.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the cytoplasmic region (amino acids 960–1161) of human MET, which contains kinase domain sequences; this region is highly conserved across human and rat, supporting the observed cross-reactivity.
• For Western blot analysis, lysates from cell lines known to express MET (e.g., A549, HeLa, or hepatocyte lines) or tissue samples (liver, kidney) can be used; expect bands around 156 kDa, possibly accompanied by additional bands representing glycosylated or cleaved forms.
• When performing ELISA, consider using the antibody as a capture or detection reagent in a sandwich format with a paired monoclonal antibody; optimization of coating concentration and detection conditions is advised.
• Since MET is a transmembrane receptor, enriched membrane preparations or immunoprecipitation may improve signal in WB; lysis buffers containing non-denaturing detergents (e.g., 1% Triton X-100) are recommended to preserve epitopes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD developers, providing not only this high-quality Anti-MET Rabbit Polyclonal Antibody but also a full range of companion antibody pairs, conjugated secondary antibodies, and bulk ancillary reagents designed for diagnostic assay development. Leveraging our deep expertise in oncology and signaling targets, we help diagnostic manufacturers and research laboratories secure rare specificity raw materials and technical consultation from concept to clinical validation. Partner with CamelBio to streamline your supply chain for reliable MET-related immunoassay components.