Anti-MET Rabbit Monoclonal Antibody for WB and ELISA - P08581

Core Product Specifications and Parameters

Biological Background: MET Function and Localization

• MET, also known as hepatocyte growth factor receptor (HGFR) or c-Met, is a receptor tyrosine kinase that transduces signals from the extracellular matrix upon binding its ligand HGF.
• Ligand-induced activation triggers autophosphorylation and recruitment of downstream effectors (e.g., PI3K, PLCγ, GRB2, STAT3), leading to activation of RAS-ERK, PI3K-AKT, and PLCγ-PKC pathways that coordinate proliferation, scattering, morphogenesis, and survival.
• During embryogenesis, MET signaling is essential for gastrulation, neuronal precursor migration, angiogenesis, and kidney formation; postnatally it supports wound healing, organ regeneration, and tissue remodeling.
• Subcellularly, MET is primarily located at the membrane (with a secreted form reported) and is expressed in hepatocytes, gastrointestinal epithelia, basal keratinocytes, thyroid, kidney, and brain.
• Tissue expression data show high levels in liver, gastrointestinal tract, thyroid, and kidney (PubMed:26637977). Related references: PMID:26637977
• MET acts as a receptor for Listeria monocytogenes internalin InlB, facilitating host cell entry.
• Post-translational modifications include phosphorylation, glycosylation, and ubiquitination; clinically, MET mutations, alternative splicing, and rearrangements are linked to deafness and multiple cancers.

Experimental Guidance and Technical Tips

• The immunogen spans the cytoplasmic domain (aa 960–1161); the antibody may recognize both full-length and cleaved C-terminal fragments. Consider this when analyzing WB band patterns.
• Standard reducing SDS-PAGE is recommended; non-reducing conditions may be evaluated for native conformation studies.
• For ELISA, use recombinant MET or HGF-stimulated cell lysates to confirm binding; cross-reactivity with mouse MET enables translational studies in mouse models, but validate in the relevant sample system.
• Optimal working dilutions should be determined empirically for each experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with streamlined access to high-quality IVD raw materials—from validated antibody pairs and optimized monoclonal antibodies to bulk ancillary reagents and rare target sourcing. MET is a central biomarker in cancer diagnostics and targeted therapy development; we support your MET-related projects with reliable, ready-to-use raw materials and flexible supply solutions. Reach out to explore how our integrated sourcing platform can accelerate your assay development from concept to clinic.