Anti-MEST Rabbit Polyclonal Antibody for WB, IF/ICC, and ELISA - Q5EB52

Core Product Specifications and Parameters

Biological Background: MEST Function and Localization

• MEST (Mesoderm-specific transcript homolog protein), also known as paternally-expressed gene 1 protein (PEG1), is encoded by the MEST gene in humans.
• MEST plays a critical role in neuronal migration during brain development by facilitating the transition of neurons from bipolar to multipolar morphologies, likely through modulation of N-cadherin-dependent cell adhesion and Akt/Wnt signaling pathways.
• It is essential for the development and maintenance of meso-diencephalic dopaminergic (mdDA) neurons.
• MEST negatively regulates the canonical Wnt signaling pathway during adipogenic differentiation by inhibiting glycosylation, maturation, and membrane localization of the Wnt coreceptor LRP6.
• The protein localizes to the endoplasmic reticulum membrane.
• MEST is a glycoprotein with a predicted molecular weight of ~39 kDa (observed) / 38.8 kDa (calculated). It contains a transmembrane domain.
• Tissue expression analysis shows high expression in hydatidiform moles; biallelic expression in lymphocytes, with isoform-specific imprinting: isoform 1 is paternal, isoform 2 is biallelic.
• Keywords: Alternative splicing, Endoplasmic reticulum, Glycoprotein, Hydrolase, Membrane, Neurogenesis.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein covering amino acids 146-335 of human MEST. This region may detect both full-length and potential isoforms.
• For western blotting, an band at approximately 39 kDa is expected; consider using appropriate positive controls (e.g., HeLa, MCF-7 lysates) given expression in human tissues.
• IF/ICC protocols may benefit from optimization of permeabilization conditions given the ER membrane localization.
• ELISA: The antibody can be used as a capture or detection reagent; validate performance in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies like this anti-MEST pAb, and bulk ancillary reagents for neurogenesis and metabolic disease studies. As a dedicated B2B partner, we source rare-target raw materials and offer technical consulting to support your assay development from concept to clinic. Rely on CamelBio for consistent, high-quality supply and expert guidance throughout your product lifecycle.