Anti-Mesothelin Rabbit Polyclonal Antibody for WB, ELISA - Q13421

Core Product Specifications and Parameters

Biological Background: Mesothelin Function and Localization

• Mesothelin is encoded by the MSLN gene and produced as a precursor protein that is cleaved into two products: the megakaryocyte-potentiating factor (MPF) and the mature membrane-bound mesothelin.
• Membrane-anchored mesothelin may play a role in cellular adhesion, potentially involved in cell-cell or cell-matrix interactions.
• Megakaryocyte-potentiating factor (MPF) acts as a cytokine that can potentiate megakaryocyte colony formation in vitro, suggesting a role in hematopoiesis.
• The protein localizes to the cell membrane via a GPI-anchor, and is also found in the Golgi apparatus and in secreted forms following cleavage and release.
• Mesothelin is expressed in mesothelial cells lining the pleura, peritoneum, and pericardium; at lower levels in lung, heart, placenta, and kidney.
• It is highly overexpressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas, making it a useful tumor marker and therapeutic target.
• Post-translational modifications include disulfide bond formation, N-linked glycosylation, and GPI-anchor attachment, which are critical for its stability and membrane association.
• Alternative splicing, phosphorylation, and proteolytic cleavage events regulate its multiple functional forms and subcellular distributions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 296–606) of human Mesothelin, which is conserved in mouse (based on cross-reactivity), making it suitable for both human and mouse sample detection in WB and ELISA.
• For Western blotting, consider that the full-length precursor (~69 kDa) is cleaved, so multiple bands may be observed depending on the sample (e.g., ~40 kDa mature mesothelin and ~31 kDa MPF). Validate with recombinant protein or lysates from high-expressing cell lines (e.g., ovarian cancer lines such as OVCAR-3 or mesothelioma lines).
• In ELISA, the antibody can be used as a capture or detection reagent; optimal coating concentration and pairing with a detection antibody should be determined empirically, ideally using recombinant Mesothelin as a standard.
• As a polyclonal antibody, batch-to-batch variability may occur; always perform a pilot test with positive and negative controls to confirm reactivity and specificity.
• Consider that endogenous soluble MPF in serum or cell culture supernatants might be detected; adjust blocking and wash conditions accordingly to reduce background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers, labs, and research institutes to provide a seamless supply of IVD raw materials, technical services, and consulting from concept to clinic. This anti-Mesothelin polyclonal antibody supports cancer and megakaryocyte research applications, and we can source or develop validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target raw materials to advance your assay development. Let CamelBio streamline your procurement process with reliable, high-quality biological reagents and expert guidance.