Anti-Mesothelin Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q13421

Core Product Specifications and Parameters

Biological Background: Mesothelin Function and Localization

• Mesothelin is a cell surface glycoprotein encoded by the MSLN gene (Gene ID: 10232) that is synthesized as a precursor and cleaved into a membrane-anchored form and a secreted megakaryocyte-potentiating factor (MPF).
• The membrane-bound form is attached via a GPI-anchor and is believed to mediate cell adhesion, potentially contributing to tumor metastasis and peritoneal dissemination.
• The secreted MPF fragment (also known as CAK1 antigen) has been shown to potentiate megakaryocyte colony formation in vitro, indicating a role in hematopoiesis.
• Mesothelin is primarily localized to the cell membrane and Golgi apparatus, with a cleaved soluble form also secreted extracellularly.
• At the protein level, mesothelin is highly expressed in mesotheliomas, ovarian cancers, and certain squamous cell carcinomas, while normal tissues show limited expression in mesothelial cells, lung, and at low levels in heart, placenta, and kidney.
• Post-translational modifications include glycosylation, disulfide bond formation, and phosphorylation; the protein also undergoes cleavage on a pair of basic residues to yield the mature forms.
• Alternative splicing of the MSLN transcript generates multiple isoforms, contributing to functional diversity.
• Mesothelin serves as a promising tumor biomarker and therapeutic target due to its differential expression pattern and involvement in cancer progression.

Experimental Guidance and Technical Tips

• The immunogen used was a recombinant fusion protein spanning amino acids 296–606 of human Mesothelin (NP_037536.2). This region covers the mature membrane-bound form, suggesting the antibody may recognize both the membrane-anchored and shed fragments.
• For Western blotting (WB), detection of endogenous mesothelin is expected at approximately 69 kDa; however, due to glycosylation and cleavage, multiple bands or a smear may be observed. Consider using deglycosylation controls.
• In immunohistochemistry (IHC-P), membrane staining should be anticipated in mesothelial and tumor tissues. Nuclear or cytoplasmic staining should be interpreted cautiously.
• For ELISA applications, pairing this antibody with a detection antibody targeting a non-overlapping epitope may help avoid competition. Validate performance in the relevant sample system using recombinant protein or tumor cell lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories streamlined access to validated raw materials for mesothelin-targeted assay development. Our catalog includes monoclonal antibodies, optimized antibody pairs, and ancillary reagents, with the capability to source rare targets and support projects from concept to clinic. Contact our team to discuss custom bulk orders or technical consultations.