Anti-Mesothelin Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - Q13421

Core Product Specifications and Parameters

Biological Background: Mesothelin Function and Localization

• Mesothelin, also known as CAK1 antigen or pre-pro-megakaryocyte-potentiating factor, is encoded by the MSLN gene and exists as multiple isoforms via alternative splicing.
• The protein is synthesized as a precursor that is cleaved into a membrane-anchored GPI-linked mature mesothelin and a secreted megakaryocyte-potentiating factor (MPF).
• Membrane-anchored mesothelin is thought to play a role in cell adhesion, potentially mediating interactions between mesothelial cells and other cell types.
• MPF, the secreted fragment, was initially identified for its ability to potentiate megakaryocyte colony formation in vitro.
• Subcellularly, mesothelin localizes to the cell membrane via its GPI anchor, as well as to the Golgi apparatus during biosynthesis; the MPF fragment is secreted extracellularly.
• Tissue expression is most prominent in lung and mesothelial cells, with lower levels in heart, placenta, and kidney; at the protein level, it is highly overexpressed in mesotheliomas, ovarian cancers, and certain squamous cell carcinomas.
• Key post-translational modifications include N-linked glycosylation, disulfide bond formation, phosphorylation, and the GPI-anchor attachment, all contributing to its structural and functional properties.
• The UniProt keywords highlight its involvement in cell adhesion, its membrane association, and its disease relevance through aberrant expression in cancers.

Experimental Guidance and Technical Tips

• The immunogen was designed against amino acids 296–606 of human Mesothelin, covering the extracellular domain; this region may be partially shared with the MPF fragment, potentially allowing detection of both forms.
• For Western blotting, a band around 69 kDa is expected for the full-length precursor or membrane-anchored form; additional bands around 30–40 kDa might represent the shed MPF fragment.
• When performing IHC-P, consider antigen retrieval at pH 6 or 9 and validate staining specificity using mesothelial or ovarian cancer tissue sections known to express high Mesothelin.
• For IF-P, fix cells with paraformaldehyde and permeabilize appropriately; confirm signal localization at the cell membrane and Golgi apparatus.
• Validate reactivity with human, mouse, and rat samples using known positive and negative cell lines; e.g., OVCAR-3 (human ovarian cancer) or AB22 (mouse mesothelioma) for positive controls.
• ELISA applications may benefit from testing both capture and detection formats with recombinant Mesothelin/MPF protein as a standard.
• Note that the antibody may detect both membrane-bound and soluble forms; consider sample preparation (cell lysate vs. conditioned medium) to distinguish them.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers IVD developers and research institutes with a streamlined path from concept to clinic, providing diagnostic-grade raw materials like this anti-Mesothelin polyclonal antibody for cancer and mesothelial target research. Our one-stop platform offers validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets, ensuring you have the right tools at every stage. Whether you are developing an ovarian cancer ELISA or exploring mesothelioma biomarkers, CamelBio bridges the gap between discovery and diagnostic realization.