Anti-Mesothelin Monoclonal Antibody for WB, IHC-P, ELISA - Q13421

Core Product Specifications and Parameters

Biological Background: Mesothelin Function and Localization

• Mesothelin is a cell surface glycoprotein encoded by the MSLN gene, also known as MPF (megakaryocyte-potentiating factor) or CAK1 antigen.
• The full-length protein is synthesized as a 71 kDa precursor that undergoes proteolytic cleavage to yield a 31 kDa N-terminal secreted form (megakaryocyte-potentiating factor, MPF) and a 40 kDa C-terminal membrane-bound mesothelin.
• Membrane-anchored mesothelin is attached to the cell surface via a glycosylphosphatidylinositol (GPI) anchor and is implicated in cell adhesion.
• MPF is secreted and has been shown to potentiate megakaryocyte colony formation in vitro, suggesting a role in hematopoiesis.
• Mesothelin is predominantly expressed in mesothelial cells lining the pleura, pericardium, and peritoneum, and is also found at lower levels in lung, heart, placenta, and kidney.
• It is highly overexpressed in several human cancers, including mesotheliomas, ovarian cancers, and some squamous cell carcinomas, making it a target for cancer diagnostics and therapeutics.
• The protein undergoes several post-translational modifications, including glycosylation, disulfide bond formation, and phosphorylation, which are critical for its stability and function.
• Subcellularly, mesothelin localizes to the cell membrane, the Golgi apparatus, and is also found in secreted forms.

Experimental Guidance and Technical Tips

• The immunogen encompasses the C-terminal region (amino acids 296–606) of human mesothelin, which corresponds to the mature membrane-bound form; therefore, the antibody is expected to detect both the full-length precursor and the cleaved membrane-bound subunit in Western blotting.
• For Western blot, consider using reducing conditions, as disulfide bonds may affect migration; the predicted molecular weight of the mature membrane mesothelin is approximately 40 kDa, but bands at higher molecular weights may be observed due to glycosylation.
• In immunohistochemistry (IHC-P), optimize antigen retrieval and validate in mesothelial or tumor tissue sections known to overexpress mesothelin, such as mesothelioma or ovarian cancer biopsies.
• For ELISA, pair with a capture antibody targeting a non-overlapping epitope to assess mesothelin levels in serum or cell culture supernatants; note that the soluble MPF form may also be detected if the epitope is present in the N-terminal region.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers a streamlined solution for sourcing high-quality IVD raw materials. From validated antibody pairs targeting mesothelin for oncology diagnostics to optimized monoclonal and polyclonal antibodies, we provide reliable reagents to support your assay development. Our extensive catalog also includes bulk ancillary reagents and access to rare-target raw materials, simplifying your path from concept to clinic.