Anti-MERS-CoV Spike RBD Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - K9N5Q8

Core Product Specifications and Parameters

Biological Background: MERS-CoV Spike Glycoprotein Function and Localization

• The spike glycoprotein (S) mediates viral entry by binding to host receptor DPP4 (dipeptidyl peptidase 4) and facilitating membrane fusion (UniProt K9N5Q8).
• It is a class I viral fusion protein; during fusion, its coiled-coil regions form a trimer-of-hairpins structure that drives membrane apposition and fusion.
• The protein is proteolytically cleaved into S1 and S2 subunits; the receptor-binding domain (RBD) resides within S1, while the fusion peptide in S2 is unmasked upon endosomal cleavage.
• Encoded by the S gene, it is a major virulence factor and the primary target of neutralizing antibodies (UniProt keywords: Virulence, Viral envelope protein).
• Subcellularly, it localizes to the virion membrane, host endoplasmic reticulum-Golgi intermediate compartment membrane, and host cell membrane (UniProt SUBCELLULAR LOCATION).
• Post-translational modifications include N-glycosylation, disulfide bond formation, and palmitoylation, which are critical for protein folding and function.
• The full-length spike protein has a predicted molecular weight of approximately 149 kDa (UniProt Mol Weight: 149,411 Da).

Experimental Guidance and Technical Tips

• The immunogen (aa 18-725 of the RBD) covers the receptor-binding domain critical for host DPP4 interaction; this antibody may be suitable for applications studying RBD–receptor binding.
• For Western blot, a band around 149 kDa is expected; glycosylation may cause slight mobility shifts. Consider using deglycosylation controls if ambiguous bands appear.
• Immunofluorescence experiments should account for the protein's localization to intracellular membranes and the cell surface; permeabilization protocols need optimization for intracellular detection.
• In ELISA setups, recombinant Spike RBD (e.g., YP_009047204.1) can serve as a positive control for antibody titration and binding specificity testing.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutes a complete portfolio of IVD raw materials, including validated monoclonal and polyclonal antibodies, recombinant antigens, and ancillary reagents. This anti-MERS-CoV Spike RBD polyclonal antibody exemplifies our capability to supply specialized tools for coronavirus research and assay development. For bulk sourcing, custom antibody generation, or rare-target reagent procurement, CamelBio supports your project from concept to clinic with efficient one-stop solutions.