Anti-MERS-CoV Nucleoprotein Rabbit Monoclonal Antibody for WB, ELISA - K9N4V7

Core Product Specifications and Parameters

Biological Background: MERS-CoV Nucleoprotein Function and Localization

• Also known as Nucleocapsid protein (NC, Protein N), the MERS-CoV nucleoprotein is encoded by the N gene.
• Packages the positive-strand viral RNA genome into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M.
• Enhances the efficiency of subgenomic viral RNA transcription as well as viral replication.
• Subcellular localization includes the virion, host endoplasmic reticulum-Golgi intermediate compartment, and host Golgi apparatus.
• Classified under keywords such as 3D-structure, ADP-ribosylation, phosphoprotein, ribonucleoprotein, RNA-binding, transcription, transcription regulation, viral nucleoprotein, and virion.
• Predicted molecular weight is approximately 45 kDa (UniProt: 44857 Da).

Experimental Guidance and Technical Tips

• The immunogen covers the full-length nucleoprotein (aa 1-413); consider that the antibody may detect both full-length protein and potential degradation fragments in WB.
• For Western blot, expect a band near 45 kDa; always validate using appropriate positive and negative controls in your sample system.
• This antibody is suitable for both ELISA and WB; optimize dilution factors and buffer conditions for your specific assay setup.
• Confirm specificity for MERS-CoV nucleoprotein by testing against other coronavirus strains if cross-reactivity data is critical for your application.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we provide a comprehensive one-stop sourcing platform for IVD raw materials, supporting diagnostic manufacturers and research labs from concept to clinic. This anti-MERS-CoV nucleoprotein monoclonal antibody is part of our extensive catalog of validated antibodies for emerging infectious diseases, including coronavirus research. We also offer bulk ancillary reagents, antigen/antibody pair development, and customized sourcing for rare targets to accelerate your assay development and scale-up.