Anti-MERS-CoV Nucleoprotein Polyclonal Antibody for WB, ELISA - K9N4V7

Core Product Specifications and Parameters

Biological Background: MERS-CoV Nucleoprotein Function and Localization

• Nucleoprotein (N), also known as nucleocapsid protein (NC) or protein N, is a structural protein of Middle East respiratory syndrome-related coronavirus (MERS-CoV).
• It packages the positive-strand viral genomic RNA into a helical ribonucleocapsid (RNP), forming the core of the virion and protecting the viral genome.
• Plays a fundamental role during virion assembly through interactions with the viral genome and the membrane protein M, ensuring proper incorporation of the RNP into budding particles.
• Enhances the efficiency of subgenomic viral RNA transcription and viral replication, acting as a key regulator of the viral life cycle.
• Subcellular localization includes the virion, host endoplasmic reticulum-Golgi intermediate compartment, and host Golgi apparatus, facilitating its multiple roles in infection.
• Post-translational modifications and functional features include ADP-ribosylation, phosphorylation (phosphoprotein), RNA-binding activity, and involvement in transcription regulation.
• The protein is classified under keywords such as ribonucleoprotein, viral nucleoprotein, and transcription regulation, reflecting its multifaceted functions.

Experimental Guidance and Technical Tips

• The immunogen covers the full-length nucleoprotein (aa 1-413) expressed as a recombinant protein, providing broad epitope recognition; consider its suitability for detecting both native and denatured forms of the N protein.
• For Western blot (WB), a band around 45 kDa is expected; validate the antibody in your specific sample system, optimizing loading controls and blocking conditions.
• In ELISA applications, evaluate direct or capture formats for detecting MERS-CoV viral antigen; note that cross-reactivity is specific to MERS-CoV, so confirm specificity with relevant controls.
• Since the antibody is a polyclonal preparation, it may recognize multiple epitopes, offering robust signal but requiring careful titration to minimize background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop partner for IVD raw materials, supporting diagnostic manufacturers and researchers from concept to clinic. This anti-MERS-CoV nucleoprotein antibody exemplifies our ability to provide high-quality reagents for emerging infectious disease research and assay development. Explore our comprehensive portfolio, including validated antibody pairs, bulk ancillary reagents, and custom sourcing services to accelerate your diagnostic projects.