Anti-MEPE Polyclonal Antibody for WB, ELISA - Q9NQ76

Core Product Specifications and Parameters

Biological Background: MEPE Function and Localization

• MEPE (Matrix extracellular phosphoglycoprotein), also known as OF45 or osteoregulin, is a secreted extracellular matrix phosphoglycoprotein involved in biomineralization.
• Promotes renal phosphate excretion and inhibits intestinal phosphate absorption. Related references: PMID:14962809 PMID:19005008
• Promotes bone mineralization by osteoblasts and cartilage mineralization by chondrocytes. Related references: PMID:18162525 PMID:19998030 PMID:22766095
• Regulates the mineralization of the extracellular matrix of the craniofacial complex, including teeth, bone, and cartilage (By similarity).
• Promotes dental pulp stem cell proliferation and differentiation. Related references: PMID:22341070
• Detected in urine and expressed by osteoblasts, dental pulp stem cells, mesenchymal cells in dental papilla and pulp, and odontoblasts.
• Localizes to the extracellular space and extracellular matrix; secreted protein.
• Subject to post-translational modifications: glycoprotein, phosphoprotein, proteoglycan.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 90-256 of human MEPE, a region likely to produce antibodies recognizing multiple epitopes due to the polyclonal nature.
• For Western blot (WB), the expected molecular weight is approximately 58 kDa. Validate using appropriate positive controls such as osteoblast or dental pulp cell lysates.
• For ELISA, consider using the recombinant MEPE protein as a standard and coating antigen; optimal working concentrations should be determined experimentally.
• Cross-reactivity is indicated for human, mouse, and rat; verify in your specific sample matrix if species other than those listed are used.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw material procurement, providing validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. For researchers focused on MEPE—a key regulator in bone and dental mineralization—we offer reliable sourcing of rare-target antibodies and related raw materials to support your assay development and production needs. We bridge the gap from concept to clinic with comprehensive technical services and consulting, ensuring a seamless supply chain for your diagnostic projects.