Anti-MEPCE Rabbit Polyclonal Antibody for IF/ICC and ELISA - Q7L2J0

Core Product Specifications and Parameters

Biological Background: MEPCE Function and Localization

• MEPCE (7SK snRNA methylphosphate capping enzyme), also known as Bicoid-interacting protein 3 homolog (Bin3 homolog), is a S-adenosyl-L-methionine-dependent methyltransferase.
• It catalyzes the addition of a methylphosphate cap to the 5'-end of 7SK snRNA, stabilizing this non-coding RNA. Related references: PMID:17643375, PMID:19906723, PMID:30559425
• MEPCE has a non-enzymatic function as a core component of the 7SK ribonucleoprotein (RNP) complex, which sequesters P-TEFb to inhibit RNA polymerase II phosphorylation and transcriptional elongation. Related references: PMID:17643375
• The 7SK RNP complex also promotes snRNA gene transcription by interacting with the little elongation complex (LEC). Related references: PMID:28254838
• MEPCE is essential for 7SK RNA stability and 7SK RNP assembly; interaction with LARP7 occludes its catalytic center. Related references: PMID:19906723, PMID:38100593
• Additionally, MEPCE is required for the stability of U6 small nuclear RNAs. Related references: PMID:38100593
• The protein localizes to the nucleus and is expressed tissue-specifically in chronic myeloid leukemia cells, adrenal gland, brain, cerebellum, kidney, lung, mammary gland, and testis. Related references: PMID:12358911
• MEPCE undergoes post-translational modifications including acetylation, methylation, phosphorylation, and ubiquitin-like conjugation, and is subject to alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 470-689 at the C-terminus of human MEPCE, a region that may be present in full-length and certain alternatively spliced isoforms.
• For immunofluorescence/immunocytochemistry (IF/ICC), optimization of fixation and permeabilization protocols is recommended due to nuclear localization of the target.
• ELISA assays may require appropriate controls and dilution optimization for specific sample types.
• Consider validating the antibody performance in your relevant cell lines or tissues, particularly those with known MEPCE expression.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutions with streamlined access to high-quality IVD raw materials, technical support, and sourcing solutions. This Anti-MEPCE antibody supports research into transcriptional regulation and snRNA biology, and our team can assist in sourcing validated antibody pairs, bulk ancillary reagents, or custom polyclonal antibody generation to meet your specific needs.