Anti-Menin Polyclonal Antibody for WB, ELISA - O00255

Core Product Specifications and Parameters

Biological Background: Menin Function and Localization

• Menin, encoded by the MEN1 gene (alternative names: MEAI, SCG2), is a ~68 kDa nuclear protein that plays a critical role in chromatin modification and gene expression.
• It serves as an essential component of the MLL/SET1 histone methyltransferase complex, which specifically methylates lysine 4 of histone H3 (H3K4), a mark associated with active transcription.
• As a transcriptional regulator, menin binds the TERT promoter to repress telomerase expression and modulates the activity of key transcription factors including JUND, RELA, and SMAD3.
• Menin positively regulates HOX gene family members (e.g., HOXC8, HOXC6, HOXA9), indicating its involvement in developmental processes and hematopoiesis, and may also participate in DNA repair.
• The protein is located in the nucleus and is ubiquitously expressed across human tissues.
• Post-translational modifications include phosphorylation, and alternative splicing generates multiple isoforms; mutations in MEN1 are linked to multiple endocrine neoplasia type 1.
• UniProt keywords: Chromatin regulator, DNA-binding, Repressor, Transcription, Transcription regulation, Phosphoprotein, Alternative splicing, Disease variant.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 266–615 of human Menin; this relatively large fragment may enable recognition of full-length and possibly some splice variants. For Western blot (WB), a principal band at ~68 kDa is anticipated in human and mouse cell/tissue lysates.
• For ELISA applications, consider using the antibody as a capture or detection reagent in indirect or sandwich formats; pairing with another Menin-specific antibody may enhance sensitivity.
• Appropriate positive and negative controls should be included: lysates from cell lines or tissues with known Menin expression (e.g., HEK293, HeLa, mouse spleen) versus knockout or knockdown models.
• Since cross-reactivity with mouse is reported, this antibody is suitable for studies in mouse models; always validate in the specific sample system before quantitative assays.
• Optimize antibody dilution for each application and detection system; pre-testing with a dilution series is recommended.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to IVD raw materials. Our Menin antibody supports research in epigenetics, transcriptional regulation, and oncology, facilitating assay development for chromatin-related targets.
• Beyond this antibody, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and sourcing for rare targets—empowering you to efficiently build robust IVD workflows.