Anti-MEMO1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9Y316

Core Product Specifications and Parameters

Biological Background: MEMO1 Function and Localization

• MEMO1 (Mediator of ErbB2-driven cell motility 1), also known as C21orf19-like protein or HCV NS5A-transactivated protein 7, is encoded by the MEMO1 gene (alternative names: C2orf4, MEMO, NS5ATP7).
• The protein functions as a mediator of ERBB2 signaling, playing a key role in relaying extracellular chemotactic signals to the microtubule cytoskeleton during cell migration.
• MEMO1 participates in the MEMO1-RHOA-DIAPH1 signaling pathway, which is essential for ERBB2-dependent stabilization of microtubules at the cell cortex.
• It regulates the localization of APC and CLASP2 to the cell membrane by modulating GSK3B activity, enabling membrane-bound APC to recruit MACF1 for microtubule capture and stabilization.
• MEMO1 is required for breast carcinoma cell migration, highlighting its significance in cancer biology.
• The protein undergoes alternative splicing and is classified as a phosphoprotein, as identified by proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen encompasses the N-terminal region (aa 1-297) of human MEMO1, which is conserved across all known isoforms, suggesting broad reactivity.
• For Western blot (WB), consider testing lysates from human, mouse, or rat cell lines, and expect a specific band at approximately 34 kDa.
• In immunohistochemistry (IHC-P), antigen retrieval may enhance staining; we recommend evaluating citrate buffer (pH 6.0) as a starting condition.
• For ELISA, standard direct or sandwich assay formats can be explored, with optimization of coating concentrations and detection systems.
• As a polyclonal antibody, batch-to-batch consistency should be validated in the user’s specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with seamless access to IVD raw materials, technical services, and consulting—supporting projects from concept to clinic. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies (such as this MEMO1 antibody), bulk ancillary reagents, and specialized sourcing for rare targets in cell migration and oncology research. Partner with CamelBio to streamline your supply chain and accelerate assay development.