Anti-Melanocortin Receptor 3 (MC3R) Rabbit Polyclonal Antibody for WB and ELISA - P41968

Core Product Specifications and Parameters

Biological Background: MC3R Function and Localization

• MC3R is a member of the melanocortin receptor family, a class of G protein-coupled receptors.
• Functions as a receptor for melanocyte-stimulating hormones (α-, β-, γ-MSH) and adrenocorticotropic hormone (ACTH), coupling to G(s) to activate adenylate cyclase and elevate cAMP levels.
  Related references: PMID:37524700, PMID:8463333, PMID:18231126
• Plays a critical role in the regulation of energy homeostasis, including control of food intake and body weight.
• Required for the expression of anticipatory activity patterns and wakefulness during limited nutrient access, and contributes to circadian clock regulation in the brain.
• Activity is antagonized by Agouti-related protein (AGRP), which blocks α-MSH-induced cAMP production.
  Related references: PMID:9311920
• Localizes to the cell membrane and is expressed in brain, placental, and gut tissues.
• Contains structural features typical of GPCRs, including disulfide bonds, palmitoylation sites, and glycosylation, and binds calcium.

Experimental Guidance and Technical Tips

• The immunogen used spans amino acids 1–120 of human MC3R; consider aligning with your target epitope for optimal recognition.
• Suitable for Western blot (detects a band at ~36 kDa) and ELISA; validate detection conditions with your specific sample types and experimental setups.
• Reported cross-reactivity with mouse and rat suggests utility for rodent models, but independent verification is recommended.
• Due to the GPCR nature, membrane protein extraction may enhance detection in WB.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutions with a one-stop supply chain for IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies like this anti-MC3R antibody. We support metabolism and circadian rhythm research with customizable reagent sourcing, bulk ancillary materials, and consulting to accelerate your assay development from concept to clinic.