Anti-Melan-A/MART-1 Polyclonal Antibody for WB, IF/ICC, ELISA - Q16655

Core Product Specifications and Parameters

Biological Background: Melan-A/MART-1 Function and Localization

• Melan-A/MART-1 is a transmembrane protein essential for melanosome biogenesis, ensuring the stability of the GPR143 receptor.
• It plays a critical role in the expression, stability, trafficking, and processing of the melanocyte protein PMEL, which is required for the formation of stage II melanosomes.
• Subcellular localization includes the endoplasmic reticulum membrane, Golgi apparatus, trans-Golgi network membrane, and melanosomes.
• Expression is highly restricted to melanoma and melanocyte cell lines, as well as the retina.
• The protein undergoes phosphorylation, as indicated by proteomics identification and keyword annotations.
• Structural studies (3D-structure keyword) have provided insights into its conformation, supporting its role as a reference proteome member.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fragment covering amino acids 48–118; consider verifying detection of endogenous full-length Melan-A/MART-1 in your sample system.
• For Western blot, note that the predicted protein weight is ~13 kDa; use an appropriate gel percentage for low-molecular-weight proteins.
• In IF/ICC, expect localization to melanosomes and Golgi-derived compartments; positive control samples from melanoma cell lines are recommended.
• Cross-reactivity with mouse and rat orthologs has been reported; validate in the relevant species model if needed.
• ELISA can be explored for quantitative detection, but optimal antibody dilutions and conditions should be determined empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal antibodies like this anti-Melan-A/MART-1, and bulk ancillary reagents to support melanoma and melanosome research. We streamline procurement from concept to clinic, ensuring reliable access to rare targets and technical consulting for diagnostic development.