Anti-MEK6 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P52564

Core Product Specifications and Parameters

Biological Background: MEK6 Function and Localization

• MEK6 (MAP2K6) is a dual specificity protein kinase essential in the MAP kinase signal transduction pathway, activating p38 MAPKs (MAPK11, MAPK12, MAPK13, MAPK14) via phosphorylation of threonine and tyrosine residues.
• Along with MAP2K3/MKK3, MEK6 mediates cellular responses to cytokines and environmental stresses, with a critical role in TNF-induced MAPK11 activation.
• MEK6 phosphorylates and activates PAK6, and its downstream p38 pathway targets transcription factors ATF2 and ELK1, regulating gene expression in response to IL-12, IL-6, and IFNG.
• Isoform 1 is expressed in skeletal muscle, heart, and to a lesser extent liver and pancreas; isoform 2 is specific to skeletal muscle.
• Subcellular localization is predominantly nuclear and cytoplasmic, with association to the cytoskeleton.
• The protein features acetylation, ATP-binding, and phosphorylation sites, and is classified under serine/threonine- and tyrosine-protein kinases, stress response, transcription regulation, and apoptosis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length recombinant human MEK6 (amino acids 1-334); verify that the antibody recognizes the target in your specific sample system.
• Recommended applications include WB (predicted band ~37 kDa), IF/ICC, and ELISA; validate dilution and workflow for each application under your assay conditions.
• Cross-reactivity with mouse and rat MEK6 is indicated; consider using appropriate positive controls for each species.
• For functional studies, note that MEK6 activation depends on upstream stress signals; ensure proper cell stimulation (e.g., TNF, IL-12) in your model.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive one-stop sourcing of IVD raw materials, supporting your MEK6-related stress signaling and kinase assay development. We provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing to streamline your diagnostic pipeline. Partner with us to accelerate your research from concept to clinic with reliable, scalable supply.