Anti-MEK5 Monoclonal Antibody for WB and ELISA - Q13163

Core Product Specifications and Parameters

Biological Background: MEK5 Function and Localization

• MEK5 is a dual-specificity mitogen-activated protein kinase kinase 5 (MAP2K5), also known as MAPK/ERK kinase 5 (MEK5), encoded by the MAP2K5 gene.
• It acts as a scaffold for a ternary complex including MAP3K2/MAP3K3, MAP3K5, and MAPK7, activating the MAPK7/ERK5 pathway.
• This pathway is critical for protecting cells from stress-induced apoptosis, promoting neuronal survival, cardiac development, and angiogenesis.
• In cardiomyocytes, MEK5 negatively regulates apoptosis by facilitating STUB1/CHIP-mediated ubiquitination and degradation of ICER-type CREM isoforms.
• MEK5 is widely expressed in adult tissues, with particularly high abundance in heart and skeletal muscle.
• As a kinase, MEK5 possesses ATP-binding activity and undergoes phosphorylation (phosphoprotein) and acetylation, regulating its function.
• Alternative splicing produces multiple isoforms of MEK5.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 250-350 of human MEK5, a region that may be conserved across species; thus, cross-reactivity with mouse and rat is expected. Validate in your specific sample system.
• For Western blot (WB), the predicted molecular weight of MEK5 is ~50 kDa (calculated from sequence: 50,112 Da). Use appropriate gel percentage and positive controls (e.g., heart or skeletal muscle lysates).
• For ELISA, consider using the antibody as capture or detection in a sandwich pair, optimizing with recombinant MEK5 protein or cell lysates.
• Since MEK5 is a kinase involved in signaling, treatment with stimuli or inhibitors may affect detection; plan experiments accordingly.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For targets like MEK5 involved in MAPK signaling and cardiac biology, we offer validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents to accelerate your assay development and research. Contact us for rare target raw-material sourcing tailored to your project needs.