Anti-MEK2 Rabbit Monoclonal Antibody (KO Validated) for WB and ELISA - P36507

Core Product Specifications and Parameters

Biological Background: MEK2 Function and Localization

• Dual specificity mitogen-activated protein kinase kinase 2 (MEK2), also known as MAP2K2, MKK2, PRKMK2, and ERK activator kinase 2, is a serine/threonine/tyrosine kinase that plays a central role in the MAPK/ERK signaling cascade.
• Catalyzes the phosphorylation of Thr and Tyr residues within the Thr-Glu-Tyr motif of MAP kinases, leading to activation of ERK1 and ERK2, thereby regulating cell proliferation, differentiation, and survival.
• Activates BRAF in a KSR1 or KSR2-dependent manner, releasing inhibitory intramolecular interactions and promoting KSR1/2-BRAF dimerization, which is essential for BRAF activation. Related references: PMID:29433126
• Localizes primarily to the cytoplasm and can associate with the membrane, consistent with its role in signaling from cell surface receptors.
• Post-translational modifications include acetylation and phosphorylation, which modulate kinase activity and interactions; the protein is also directly sequenced and identified by proteomics.
• Keywords from UniProt indicate involvement in diseases such as cardiomyopathy, ectodermal dysplasia, and intellectual disability, highlighting the importance of MEK2 in developmental and cardiac processes.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide covering the N-terminal region (amino acids 1–100) of human MEK2. This region shows sequence conservation with mouse, consistent with the reported cross-reactivity.
• For Western blotting, whole cell lysates from human or mouse cell lines (e.g., HeLa, NIH/3T3) are recommended; a band at ~44 kDa is expected. Validate in your specific sample type and optimize antibody dilution.
• For ELISA, ensure proper coating with the immunizing peptide or recombinant MEK2 protein. Include appropriate controls and consider testing multiple antibody concentrations to achieve optimal signal-to-noise ratio.
• Knockout-validated status confirms specificity; however, always confirm target knockdown efficiency in your experimental system to avoid false negatives/positives.

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CamelBio is your one-stop IVD raw material sourcing partner, dedicated to accelerating diagnostic development from concept to clinic. Our comprehensive portfolio includes validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents for targets like MEK2 in MAPK signaling and oncology. Whether you need this high-quality anti-MEK2 antibody or support in sourcing rare raw materials, CamelBio provides reliable supply solutions tailored to your research and manufacturing needs.