Anti-MEK1/MEK2 Rabbit Polyclonal Antibody for WB, ELISA - Q02750/P36507

Core Product Specifications and Parameters

Biological Background: MEK1/MEK2 Function and Localization

• MEK1 (MAP2K1) is a dual-specificity protein kinase that plays a central role in the mitogen-activated protein kinase (MAPK) signaling cascade, specifically in the ERK pathway. Upon activation by RAF1, it phosphorylates both threonine and tyrosine residues in MAPK3/ERK1 and MAPK1/ERK2.
• MEK1 and its close homolog MEK2 (MAP2K2) are functionally overlapping and cooperate to transduce signals from cell surface receptors to nuclear and cytoplasmic effectors, regulating diverse processes including cell proliferation, differentiation, survival, and adhesion.
• MEK1 interacts with scaffold proteins KSR1 or KSR2 to facilitate BRAF activation, releasing an inhibitory intramolecular interaction that promotes KSR-BRAF dimerization and downstream signaling (PubMed:29433126). Related references: PMID:29433126
• MEK1 influences peroxisome proliferator-activated receptor gamma (PPARG) by mediating its nuclear export, thereby modulating PPARG-dependent transcription and differentiation/apoptosis.
• Subcellularly, MEK1 is predominantly cytoplasmic but also found at the centrosome, spindle poles, nucleus, and membrane, reflecting its dynamic roles in mitotic regulation, endosomal trafficking, and nuclear signaling.
• The kinase is widely expressed across tissues, with notably low levels in the brain, and post-translational modifications including acetylation and phosphorylation are critical for its regulation.
• Mutations in MAP2K1 are associated with human developmental disorders such as cardiofaciocutaneous syndrome and conditions including cardiomyopathy, ectodermal dysplasia, and intellectual disability.

Experimental Guidance and Technical Tips

• Since this polyclonal antibody is raised against an internal peptide of human MAP2K1, it may detect both MEK1 and MEK2 due to sequence similarity, as well as mouse and rat orthologs. Validate specificity in your model system.
• For Western blotting, using reducing conditions and whole-cell lysates, expect bands around 43-44 kDa for MEK1 and 45 kDa for MEK2; additional bands may reflect post-translational modifications or degradation products.
• For ELISA, the antibody can be used as capture or detection reagent; titrate and optimize for direct or indirect assay formats.
• Include appropriate positive and negative controls (e.g., stimulated vs unstimulated cells) to confirm pathway-dependent detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted partner for IVD raw-material procurement, offering one-stop access to high-quality antibodies, antigens, and reagents for diagnostic development. For MEK1/MEK2 research and assay applications, we supply well-characterized polyclonal antibodies like this one, along with complementary materials such as recombinant proteins and matched antibody pairs, ensuring comprehensive support from concept to clinic.