Anti-MEK1/MEK2 Monoclonal Antibody for WB, IHC-P, ELISA - Q02750 / P36507

Core Product Specifications and Parameters

Biological Background: MEK1/MEK2 Function and Localization

• MEK1 (MAP2K1) and MEK2 (MAP2K2) are dual-specificity protein kinases that serve as critical components of the MAPK/ERK signaling cascade, transmitting signals from activated RAS to ERK.
• They catalyze the phosphorylation of threonine and tyrosine residues in the Thr-Glu-Tyr motif of MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and propagation of downstream signals involved in cell growth, adhesion, survival, and differentiation.
• MEK1 activates BRAF in a KSR1- or KSR2-dependent manner, relieving inhibitory intramolecular interactions to promote BRAF dimerization and activation. Related references: PMID:29433126
• Subcellularly, MEK1 is found in the cytoplasm, including centrosomes and spindle poles, and can shuttle to the nucleus and membrane, reflecting its dynamic role in signal transduction.
• MEK1 is widely expressed across tissues, with particularly low levels in the brain.
• The protein is subject to multiple post-translational modifications including phosphorylation and acetylation, and it harbors kinase domains that bind ATP and nucleotide substrates.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide mapping to amino acids 170–270 of human MEK1 and MEK2, a region likely within the kinase domain; consider verifying epitope specificity by peptide competition or mutagenesis.
• For Western blot, use whole-cell lysates from human, mouse, or rat cell lines; a specific band near 44 kDa is expected. Optimize blocking and antibody dilution for low-background detection.
• For IHC-P, antigen retrieval (e.g., citrate buffer, pH 6.0) and titration of primary antibody on known positive control tissues (e.g., colon carcinoma) are recommended.
• For ELISA, the antibody may be employed as a capture or detection reagent; validate pairing with suitable antigen standards and cross-reactivity with MEK1/MEK2 paralogs.
• Confirm target specificity using MEK1/MEK2 knockout or knockdown models in the relevant species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop procurement partner for IVD raw materials, offering validated monoclonal antibodies, antibody pairs, and bulk ancillary reagents. For researchers studying the MAPK/ERK signaling axis, CamelBio ensures reliable access to this anti-MEK1/MEK2 antibody, along with a comprehensive portfolio of related signaling reagents and rare-target sourcing capabilities to accelerate diagnostic development from concept to clinic.