Anti-MEK1/MEK2 Monoclonal Antibody for WB, ELISA - Q02750/P36507

Core Product Specifications and Parameters

Biological Background: MEK1/MEK2 Function and Localization

• MEK1 (MAP2K1) and MEK2 (MAP2K2) are closely related dual-specificity kinases that serve as essential components of the mitogen-activated protein (MAP) kinase signal transduction pathway.
• In the MAPK/ERK cascade, MEK1/2 are activated by RAF kinases and subsequently phosphorylate ERK1/2 on threonine and tyrosine residues within a Thr-Glu-Tyr motif, leading to ERK activation.
• MEK1 has been shown to activate BRAF in a KSR1 or KSR2-dependent manner, releasing inhibitory intramolecular interactions and promoting BRAF dimerization. Related references: PMID:29433126
• The pathway mediates diverse biological functions such as cell growth, adhesion, survival, and differentiation, primarily through regulation of transcription, metabolism, and cytoskeletal rearrangements.
• MEK1 exports the nuclear receptor PPARG from the nucleus, linking the cascade to nuclear receptor signaling.
• The MAPK/ERK cascade is also involved in regulation of endosomal dynamics, including lysosome processing and endosome cycling, and contributes to Golgi fragmentation during mitosis.
• MEK1 is widely expressed in human tissues, with extremely low levels in the brain.
• Subcellularly, MEK1 localizes to the cytoplasm, cytoskeleton, centrosome, spindle pole, nucleus, and membrane.
• Post-translational modifications include phosphorylation and acetylation, and alternative splicing gives rise to multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–360) of human MEK1, a sequence highly conserved in MEK2; this suggests the antibody may recognize both kinases. Validate specificity using relevant knockout or knockdown controls.
• For Western blot, expect a band around 44 kDa. Optimize loading amounts and antibody dilutions for your specific sample type (e.g., whole-cell lysates) and include appropriate positive (e.g., HeLa, A431) and negative controls.
• This antibody is reported to cross-react with mouse and rat. Confirm reactivity in your experimental system when working with these species.
• For ELISA applications, consider pairing with a suitable detection antibody for sandwich ELISA development; titer optimization may be necessary.
• Due to the critical role of MEK1/2 in cell signaling, treatment with pathway activators/inhibitors (e.g., growth factors, U0126) can be used to modulate signal intensity in your assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement for diagnostic manufacturers and research teams, offering one-stop access to high-quality antibodies, validated antibody pairs, and bulk ancillary reagents. This anti-MEK1/MEK2 monoclonal antibody supports the development of assays targeting the MAPK/ERK pathway—a key cascade in oncology and other diseases. Whether you need optimized monoclonal antibodies, technical consulting, or assistance with rare-target reagent sourcing, CamelBio provides reliable supply solutions to accelerate your project from concept to clinic.