Anti-MEK1 Rabbit Monoclonal Antibody for WB, IP, ELISA - Q02750

Core Product Specifications and Parameters

Biological Background: MEK1 Function and Localization

• MEK1 (MAP2K1) is a dual specificity protein kinase that serves as a critical node in the RAS-RAF-MEK-ERK signal transduction cascade, phosphorylating both threonine and tyrosine residues on ERK1/2 (MAPK3/MAPK1) for full activation.
• The protein is widely expressed across tissues, with notably low levels in the brain, and alternative splicing generates multiple isoforms (from UniProt keywords).
• MEK1 is activated through phosphorylation by RAF kinases (RAF1, BRAF, ARAF) in response to extracellular growth factors, cytokines, and hormones binding to cell-surface receptors.
• MEK1 also functions in a scaffolding role to promote BRAF activation by binding to KSR1 or KSR2, releasing inhibitory interactions and facilitating BRAF dimerization (PubMed:29433126). Related references: PMID:29433126
• Subcellularly, MEK1 localizes to the cytoplasm, centrosome, spindle pole, nucleus, and membrane, consistent with its roles in mitosis, endosomal trafficking, and Golgi fragmentation.
• The kinase orchestrates diverse cellular processes including proliferation, differentiation, adhesion, survival, metabolism, and cytoskeletal reorganization through ERK-mediated regulation of transcription factors and nuclear receptors (e.g., PPARG nuclear export).
• Post-translational modifications such as acetylation and phosphorylation modulate MEK1 activity, and genetic variants are linked to cardiomyopathies, ectodermal dysplasia, and intellectual disability.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide covering amino acids 150–250 of human MEK1, a region within the kinase domain that is highly conserved across human, mouse, and rat, explaining the reported cross-reactivity. Consider verifying reactivity in your specific experimental system.
• For WB, expect a band near 43 kDa. Monitor gel migration as MEK1 may exhibit slight shifts upon phosphorylation or other modifications.
• IP experiments can be performed using standard protocols; optimize antibody-to-lysate ratios and incubation conditions for your cell/tissue type of interest.
• In ELISA, this antibody can be used for direct antigen capture or detection of recombinant MEK1. Include appropriate positive and negative controls to confirm specificity.
• Given MEK1’s central role in MAPK signaling, ensure that your experimental conditions (e.g., serum starvation, stimulation) are well controlled to interpret activation-dependent effects.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a dedicated one-stop sourcing partner for IVD raw materials, connecting diagnostic manufacturers and research teams with high-quality antibodies and reagents. For MEK1 and the broader MAPK pathway, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to support your assay development from concept to clinic. Our global network ensures reliable access to rare and specialized targets, streamlining your path to commercial diagnostic kit production.