Anti-MEIS2 Monoclonal Antibody for WB, ELISA - O14770

Core Product Specifications and Parameters

Biological Background: MEIS2 Function and Localization

• MEIS2 (Meis1-related protein 1) is a homeobox protein encoded by the MEIS2 (MRG1) gene, classified as a transcription factor with a DNA-binding homeobox domain.
• Functions as a transcriptional regulator by forming complexes with HOX and PBX proteins to stabilize DNA-protein interactions, thereby modulating target gene expression.
• Isoform 3 participates in a pancreatic acinar cell-specific complex with PDX1 and PBX1b to activate the ELA1 enhancer, cooperating with PTF1.
• Isoforms 3 and 4 bind the EPHA8 promoter via the 5'-CGGTCA-3' motif, potentially with PBX proteins, to regulate midbrain development; isoform 5 lacks DRD1 activation ability.
• Localizes primarily to the nucleus but also found in the cytoplasm and perinuclear region, consistent with its roles in transcription.
• Expressed ubiquitously with high levels in lymphoid organs and specific brain regions like the putamen, suggesting roles in hematopoiesis and neural function.
• Implicated in developmental processes and associated with intellectual disability as a disease variant, reflecting its importance in neurodevelopment.
• Keywords include alternative splicing, developmental protein, DNA-binding, and reference proteome, highlighting its complex regulatory roles.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 1–100 of human MEIS2, targeting the N-terminal region; consider this epitope mapping when interpreting results.
• For Western blot, the predicted molecular weight is 52 kDa; use appropriate gel percentage and validate with positive and negative controls in human and mouse samples.
• Since the antibody is validated for ELISA, it may be suitable for quantitative detection; optimize coating concentrations and detection conditions for your specific assay.
• Cross-reactivity with mouse MEIS2 suggests utility in mouse model studies; verify performance in your tissue or cell line of interest.

CamelBio: Your One-Stop Sourcing Bridge

As a one-stop IVD raw-material sourcing partner, CamelBio supports diagnostic manufacturers, laboratories, and research institutes with comprehensive access to critical reagents like the Anti-MEIS2 monoclonal antibody. This antibody serves transcription regulation and developmental biology research, and CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal formats, bulk ancillary reagents, and custom sourcing for rare targets. From concept to clinic, we bridge sourcing gaps to accelerate your diagnostic and research development.