Anti-MEIS1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O00470

Core Product Specifications and Parameters

Biological Background: Homeobox protein Meis1 Function and Localization

• MEIS1 encodes homeobox protein Meis1, a transcription factor involved in development and hematopoiesis.
• It acts as a transcriptional regulator of PAX6 and as a cofactor for HOXA7 and HOXA9, contributing to myeloid leukemia induction.
• In complex with PBX1 or PBX2, MEIS1 activates PF4 transcription, and is required for megakaryocyte lineage development and vascular patterning.
• The protein localizes to the nucleus and contains a DNA-binding homeobox domain.
• MEIS1 is expressed at low levels in normal immunohepatopoietic tissues, including fetal liver, but shows elevated expression in certain myeloid leukemia cell lines, especially those with megakaryocytic-erythroid phenotype, and in the cerebellum.
• Alternative splicing produces multiple isoforms, and the protein is subject to post-translational modifications.

Experimental Guidance and Technical Tips

• This antibody was raised against a synthetic peptide corresponding to amino acids 300-390 of human MEIS1; consider that this region is predicted to be accessible in the native protein based on sequence analysis.
• For Western blotting, a band around 43 kDa is expected; optimize primary antibody dilution starting at 1:500-1:2000 and validate with positive controls such as leukemia cell lines or cerebellar tissue lysates.
• In immunofluorescence/immunocytochemistry, nuclear staining should be observed; include appropriate fixation and permeabilization steps.
• For ELISA, the peptide immunogen can serve as a positive control; assay conditions should be optimized individually.
• Cross-reactivity with mouse and rat MEIS1 is expected due to sequence homology; however, confirm specificity in your target species by sequence alignment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This anti-MEIS1 antibody supports research in transcription regulation, hematopoiesis, and leukemia. Whether you require custom conjugation, bulk orders, or tailored supply solutions, CamelBio is equipped to streamline your transition from concept to clinic.