Anti-Megalin/LRP2 Monoclonal Antibody for WB, IF, IHC, FC and ELISA - P98164

Core Product Specifications and Parameters

Biological Background: Megalin/LRP2 Function and Localization

• Megalin/LRP2 is a 522 kDa type I transmembrane protein belonging to the LDL receptor family, also known as glycoprotein 330 (gp330), encoded by the LRP2 gene.
• It functions as a multiligand endocytic receptor, partnering with cubilin (CUBN) to mediate uptake of diverse ligands including lipoproteins, vitamin-carrier complexes, hormones, drugs, and metalloproteins.
• In the kidney, megalin mediates proximal tubular reabsorption of essential molecules: 25-hydroxyvitamin D3 (bound to GC/DBP), metallothionein-bound heavy metals, myoglobin, APOM, CST3, and MMP2/TIMP1 complexes. Related references: PMID:15126248
• It facilitates leptin transport across the blood-brain barrier and enables hypothalamic leptin signaling, regulating feeding and body weight.
• During embryogenesis, megalin is crucial for neural tube closure by mediating folate uptake (FOLR1); it also controls SHH morphogen gradient formation and BMP4 clearance in the neuroepithelium.
• In the adult brain, megalin negatively regulates BMP signaling in the subependymal zone, supporting neurogenesis; in astrocytes, it endocytoses albumin for oleic acid synthesis.
• LRP2 is expressed in absorptive epithelia (e.g., renal proximal tubules), placental trophoblasts, and neuronal projections; subcellularly it localizes to apical membranes, coated pits, endosomes, dendrites, and axons.
• Clinically, mutations in LRP2 are associated with Donnai-Barrow syndrome, featuring deafness and proteinuria; megain also mediates renal uptake of BIRC5/survivin, protecting against acute kidney injury. Related references: PMID:23825075

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 4446–4655) of human LRP2; the antibody may recognize an intracellular epitope, making it suitable for applications requiring permeabilization (e.g., IF-P, IHC-P, FC intra).
• Due to the large molecular weight (~522 kDa), for Western blotting use low-percentage SDS-PAGE gels (e.g., 6% or gradient 4–12%) and extended wet transfer times (overnight at 30 V); include a high-molecular-weight marker.
• For immunofluorescence and immunohistochemistry, validate fixation and permeabilization conditions in LRP2-expressing tissues such as kidney proximal tubules or placental villi.
• Cross-reactivity with mouse and rat has been reported; confirm in appropriate positive control lysates/tissues (e.g., rodent kidney cortex).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research labs seamless access to premium IVD raw materials, including this high-affinity recombinant monoclonal anti-Megalin/LRP2 antibody. Whether you need validated antibody pairs for sandwich immunoassays, optimized monoclonals for IHC/IF, or bulk ancillary reagents, our portfolio supports endocytosis and receptor biology projects from discovery to clinical application. With capabilities in rare target sourcing and custom development, we bridge your concept-to-clinic journey with reliable, scalable supply solutions.