Anti-MEF2C Rabbit Polyclonal Antibody for WB, IF, IHC, ELISA - Q06413

Core Product Specifications and Parameters

Biological Background: MEF2C Function and Localization

• MEF2C is a member of the myocyte enhancer factor 2 (MEF2) family of transcription factors, which play key roles in muscle-specific gene expression and development.
• It functions as a transcriptional activator by binding to the MEF2 response element in the regulatory regions of target genes, controlling cardiac morphogenesis, myogenesis, and vascular development.
• MEF2C is essential for normal neuronal development and function, including hippocampal-dependent learning and memory, and regulates excitatory synapse number and synaptic transmission.
• The protein is crucial for megakaryocyte and platelet development, bone marrow B-lymphopoiesis, B-cell survival, proliferation, and germinal center formation.
• Alternative splicing produces isoforms; isoforms lacking the repressor domain exhibit higher transcriptional activity.
• Subcellular localization includes the nucleus, cytoplasm, and sarcoplasm, reflecting its dynamic roles in transcription and signaling.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination, which modulate its activity and stability.
• Tissue expression is prominent in brain and skeletal muscle, consistent with its roles in neurogenesis and myogenesis; mutations are associated with epilepsy and intellectual disability.

Experimental Guidance and Technical Tips

• The immunogen spans a central region (aa 170-380) of human MEF2C, which is conserved across species (human, mouse, rat), supporting cross-reactivity in Western blot and immunohistochemistry.
• For Western blot, a band around 51 kDa is expected based on the calculated molecular weight; however, post-translational modifications or isoform variation may result in additional bands.
• In immunofluorescence or IHC, MEF2C localizes to the nucleus, cytoplasm, and sarcoplasm depending on cell type and fixation conditions; validate specificity using appropriate controls.
• ELISA applications may require optimizing coating antigen and antibody dilution; consider pairing with a matched secondary antibody for optimal signal.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive one-stop sourcing platform for IVD raw materials, supporting researchers and diagnostic manufacturers from concept to clinic with high-quality antibodies and reagents. This anti-MEF2C polyclonal antibody is part of our extensive portfolio for transcription factor and neurodevelopment research, and we offer validated antibody pairs, bulk antibodies, and customized sourcing for rare targets. For your MEF2C-related projects, CamelBio ensures reliable and scalable supply to accelerate your assay development.