Anti-MEF2A Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q02078

Core Product Specifications and Parameters

Biological Background: MEF2A Function and Localization

• MEF2A is a member of the myocyte enhancer factor-2 (MEF2) family of transcription factors, characterized by a MADS-box and MEF2 domain.
• It binds specifically to the MEF2 element (5'-YTAATTAR-3') in the regulatory regions of numerous muscle-specific, growth factor-induced, and stress-responsive genes.
• MEF2A plays critical roles in skeletal and cardiac muscle development, as well as in neuronal differentiation and survival.
• Through p38 MAPK signaling, MEF2A regulates cell growth, survival, and apoptosis in a tissue-specific manner.
• Alternative splicing produces multiple isoforms; isoform MEF2 and MEFA are predominantly expressed in skeletal muscle, cardiac muscle, and brain, while RSRFC4 and RSRFC9 are ubiquitously expressed.
• MEF2A localizes to the nucleus and undergoes various post-translational modifications, including phosphorylation, acetylation, sumoylation, and ubiquitin-like conjugation.
• In cerebellar granule neurons, sumoylated and phosphorylated MEF2A represses NUR77 transcription, promoting synaptic differentiation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100) of human MEF2A, which is conserved across human, mouse, and rat, consistent with the reported cross-reactivity.
• For Western blotting, the predicted molecular weight is approximately 55 kDa; however, post-translational modifications may result in observed bands at shifted positions. Consider using appropriate positive control lysates from skeletal muscle, heart, or brain tissues.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), nuclear staining is expected. Antigen retrieval and permeabilization steps should be optimized for the specific tissue or cell type.
• For ELISA, pairing with a matched detection antibody may be required; consider evaluating the antibody in a sandwich ELISA format if quantitative detection is desired.
• Always validate the antibody in the relevant sample system and include appropriate controls, as performance may vary depending on the experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive range of IVD raw materials, from optimized monoclonal and polyclonal antibodies to ancillary reagents, supporting diagnostic developers in cardiovascular and neurological research areas. As a sourcing partner, CamelBio can supply validated antibody pairs, bulk quantities, and assist in sourcing rare target reagents like MEF2A antibodies, helping streamline your assay development pipeline from concept to clinic.