Anti-MED7 Rabbit Polyclonal Antibody for WB and ELISA - O43513

Core Product Specifications and Parameters

Biological Background: MED7 Function and Localization

• MED7, also known as ARC34, CRSP9, or CRSP33, is a subunit of the Mediator complex, a large multiprotein coactivator that regulates transcription of nearly all RNA polymerase II-dependent genes.
• The Mediator complex functions as a molecular bridge, conveying regulatory information from gene-specific transcription factors to the basal RNA polymerase II transcription machinery, and facilitating the assembly of the preinitiation complex.
• MED7 is localized to the nucleus, consistent with its role in transcription regulation.
• The Mediator complex is recruited to gene promoters through direct interactions with regulatory proteins, where it serves as a scaffold for the functional preinitiation complex.
• MED7 is subject to post-translational modifications, including phosphorylation and ubiquitin-like conjugation (e.g., isopeptide bond formation), which may modulate its activity or interactions.
• The protein has a calculated molecular weight of approximately 27 kDa, as confirmed by direct protein sequencing and proteomics identification.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein spanning the N-terminal 233 amino acids of human MED7. For Western blotting, a band at ~27 kDa is expected; consider using human or mouse cell lysates as positive controls.
• Due to the polyclonal nature of this antibody, multiple epitopes on MED7 may be recognized, potentially enhancing sensitivity in applications such as ELISA.
• For ELISA, titrate both the coating antigen and primary antibody to optimize signal-to-noise ratio; consider direct or indirect ELISA formats depending on the detection system.
• When working with mouse samples, cross-reactivity is supported, but validation in the specific experimental context is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop partner for IVD raw materials, connecting diagnostic manufacturers and research labs with high-quality reagents from concept to clinic. This anti-MED7 antibody, targeting a key transcriptional coactivator, is just one example of our extensive portfolio, which also includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing services. Whether you need routine supplies or specialized materials for transcription biology, CamelBio streamlines procurement to accelerate your development.