Anti-MED6 Rabbit Polyclonal Antibody for WB and ELISA - O75586

Core Product Specifications and Parameters

Biological Background: MED6 Function and Localization

• MED6 (Mediator of RNA polymerase II transcription subunit 6) is a component of the Mediator complex, a multiprotein coactivator required for regulated transcription of nearly all RNA polymerase II-dependent genes.
• It functions as a molecular bridge, conveying regulatory signals from gene-specific activators to the basal transcription machinery, facilitating the assembly of the preinitiation complex.
• The protein is predominantly localized to the nucleus, consistent with its role in transcription regulation.
• MED6 is also known under alternative names such as Activator-recruited cofactor 33 kDa component (ARC33), Mediator complex subunit 6 (hMed6), and Renal carcinoma antigen NY-REN-28.
• Alternative splicing of the MED6 gene produces multiple transcript variants, potentially contributing to functional diversity.
• Post-translational modifications include acetylation and isopeptide bond formation (ubiquitination), which may modulate its activity or interactions.

Experimental Guidance and Technical Tips

• The antibody is raised against residues 27–246 of human MED6, encompassing the core region. It is expected to detect both full-length MED6 and potential splice variants containing this domain, if expressed.
• For Western blot, a band corresponding to approximately 28 kDa (calculated molecular weight: 28.4 kDa) is expected. Post-translational modifications such as acetylation and ubiquitination may cause minor band shifts.
• This antibody is validated for ELISA and Western blot. It may also be suitable for other applications (e.g., immunoprecipitation, immunofluorescence); however, these should be optimized and verified experimentally.
• Cross-reactivity is reported for human. If studying other organisms, validation in the relevant sample system is advised.
• As a polyclonal antibody, it provides high sensitivity but may exhibit batch-to-batch variation. Including appropriate positive and negative controls is recommended, such as cell lines with known MED6 expression levels or siRNA-mediated knockdown.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes a comprehensive one-stop platform for IVD raw materials, technical services, and strategic consulting—supporting projects from concept to clinic. This MED6 polyclonal antibody is part of our extensive portfolio of transcription and oncology research reagents. Beyond antibodies, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and can assist with sourcing rare-target raw materials to accelerate your diagnostic development.