Anti-MED4 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q9NPJ6

Core Product Specifications and Parameters

Biological Background: MED4 Function and Localization

• MED4 encodes the Mediator of RNA polymerase II transcription subunit 4, also known as ARC36, DRIP36, TRAP36, or VDRIP, and is a core component of the Mediator complex.
• The Mediator complex functions as a coactivator that bridges gene-specific regulatory proteins with the basal RNA polymerase II transcription machinery, regulating nearly all class II gene transcription.
• It serves as a scaffold for the assembly of the preinitiation complex, recruiting RNA polymerase II and general transcription factors to target gene promoters.
• MED4 localizes exclusively to the nucleus, consistent with its role in transcriptional regulation.
• Post-translational modifications, including acetylation and phosphorylation, have been identified and may modulate MED4 activity or interactions within the Mediator complex.
• Alternative splicing generates multiple MED4 isoforms, potentially contributing to functional diversity.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 150–250 of human MED4, a region likely conserved across species, supporting the observed cross-reactivity with mouse and rat.
• For Western blotting, the predicted molecular weight is approximately 30 kDa; however, confirm the band size in your specific sample system, as post-translational modifications may cause shifts.
• In immunohistochemistry (paraffin-embedded), ensure optimal antigen retrieval and titrate the antibody concentration to achieve specific staining.
• For ELISA applications, consider using this antibody as either a capture or detection reagent and validate performance with appropriate standards and sample matrices.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with streamlined access to validated IVD raw materials, including this MED4 monoclonal antibody for transcriptional regulation studies. Our sourcing capabilities encompass optimized antibody pairs, bulk ancillary reagents, and rare-target antibodies, supporting your development from concept to clinic.