Anti-MED4 Polyclonal Antibody for WB, ELISA - Q9NPJ6

Core Product Specifications and Parameters

Biological Background: MED4 Function and Localization

• MED4, also known as ARC36, DRIP36, and VDRIP, is a subunit of the Mediator complex, an essential coactivator for RNA polymerase II-mediated transcription.
• The Mediator complex bridges gene-specific regulatory proteins and the basal transcription machinery, facilitating assembly of the functional preinitiation complex (PIC) at gene promoters.
• MED4 is a core component of the Mediator, contributing to complex stability and enabling regulated expression of nearly all RNA polymerase II-dependent genes.
• The protein localizes predominantly to the nucleus, consistent with its transcriptional role, and is subject to post-translational modifications such as acetylation and phosphorylation.
• Multiple lines of evidence, including direct protein sequencing and proteomics identification, support its characterization; alternative splicing generates variant isoforms.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 1–270) covers the full-length protein; consider that the polyclonal antibody may recognize multiple epitopes and possibly related isoforms or post-translationally modified forms.
• For Western blot, the predicted molecular weight is approximately 30 kDa; validate specificity in human cell lines or tissue lysates, and use appropriate loading controls.
• In ELISA applications, optimize coating and detection conditions using the recombinant immunogen or native protein from human samples to ensure consistent performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, including validated antibodies for gene regulation and transcription studies. For research into Mediator complex biology and related gene expression mechanisms, we offer ready-to-use antibody pairs, bulk polyclonal and monoclonal antibodies, and custom sourcing of ancillary reagents. Contact us to streamline your MED4 antibody procurement and accelerate your assay development.