Anti-MED24 Polyclonal Antibody for WB and ELISA - O75448

Core Product Specifications and Parameters

Biological Background: MED24 Function and Localization

• MED24 is a subunit of the Mediator complex, a large multi-protein coactivator essential for regulated transcription by RNA polymerase II.
• The Mediator complex bridges gene-specific transcription factors with the basal transcription machinery, facilitating preinitiation complex assembly.
• MED24 is also known as ARC100, CRSP4, DRIP100, THRAP4, TRAP100, and others, reflecting its identification in various transcription coactivator complexes.
• Localized predominantly in the nucleus, MED24 functions at promoter regions to support transcription initiation.
• The protein undergoes phosphorylation and alternative splicing, producing isoforms with potentially distinct regulatory roles.
• MED24 is ubiquitously expressed, with higher abundance in skeletal muscle, heart, and placenta, suggesting roles in tissue-specific gene regulation.

Experimental Guidance and Technical Tips

• The immunogen comprises the N-terminal region (amino acids 1-160), which may influence epitope recognition; consider this when planning epitope mapping or competitive assays.
• For Western blot validation, use positive controls such as HeLa or HEK293 lysates, as MED24 is ubiquitously expressed; the predicted molecular weight is 110 kDa.
• Perform antibody titration when using ELISA, as optimal dilution may vary with antigen presentation and detection system.
• Cross-reactivity with mouse and rat MED24 is indicated; verify detection in your specific sample system if using non-human targets.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, covering every stage from concept to clinic. This MED24 antibody supports transcription regulation research, and we offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and assistance in sourcing rare target raw materials. Whether you need routine supplies or tailored solutions, CamelBio is your reliable partner for IVD development.