Anti-MED21 Polyclonal Antibody for WB, ELISA - Q13503

Core Product Specifications and Parameters

Biological Background: MED21 Function and Localization

• MED21, also known as Mediator complex subunit 21, SRB7, SURB7, or hSrb7, is a core component of the Mediator complex.
• The Mediator complex acts as a transcriptional coactivator for virtually all RNA polymerase II-dependent genes, bridging gene-specific regulatory proteins and the basal transcription machinery.
• MED21 is recruited to gene promoters via direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex.
• It localizes to the nucleus, consistent with its role in transcription regulation.
• UniProt keywords associated with MED21 include 3D-structure, activator, nucleus, reference proteome, transcription, and transcription regulation.

Experimental Guidance and Technical Tips

• The immunogen comprises the N-terminal region (amino acids 1–144) of human MED21; consider this when assessing detection of full-length versus truncated forms.
• For Western blot, the predicted molecular weight is approximately 16 kDa; include appropriate molecular weight markers.
• Validate antibody performance in the relevant sample system (e.g., human or mouse cell lysates as indicated by cross-reactivity data).
• Optimize ELISA conditions using the recombinant protein immunogen as a positive control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw-material procurement, offering a comprehensive portfolio that includes validated antibodies, bulk ancillary reagents, and support for rare target sourcing. This anti-MED21 polyclonal antibody supports research into transcriptional regulation and Mediator complex biology, and our team is ready to assist with customized sourcing strategies for your specific assay development requirements. From concept to clinic, CamelBio streamlines your supply chain with reliable, one-stop access to critical raw materials.