Anti-MED20 Polyclonal Antibody for WB, ELISA - Q9H944

Core Product Specifications and Parameters

Biological Background: MED20 Function and Localization

• MED20, also known as Mediator complex subunit 20 or TRF-proximal protein homolog (hTRFP), is a component of the Mediator complex, a large multi-subunit coactivator essential for regulated transcription by RNA polymerase II.
• The Mediator complex functions as a molecular bridge, conveying regulatory signals from gene-specific transcription factors to the basal transcription machinery, thereby facilitating the assembly of the preinitiation complex.
• MED20 is ubiquitously expressed and localizes to the nucleus, consistent with its role in transcription regulation.
• Alternative splicing of the MED20 gene produces multiple transcript variants, which may contribute to functional diversity.
• Structurally, MED20 has been characterized by 3D-structure analysis, providing insights into its interactions within the Mediator complex.
• As a reference component of the transcription apparatus, MED20 is subjected to proteomics identification and has been validated by direct protein sequencing.
• MED20 is classified as a transcriptional activator and a key reference proteome entry, highlighting its fundamental role in gene expression.

Experimental Guidance and Technical Tips

• This polyclonal antibody was raised against a recombinant protein corresponding to the N-terminal region (aa 1-212), which is predicted to be accessible in native conformation; it may recognize multiple epitopes.
• For Western blot (WB), standard denaturing conditions (e.g., sample boiling with loading buffer containing SDS and reducing agent) are recommended; the expected band size is approximately 23 kDa. Validate the antibody in a relevant sample system, considering potential splice isoforms.
• For ELISA applications, the antibody can be used as either capture or detection reagent; pairing with a monoclonal anti-MED20 antibody may enhance specificity in sandwich formats.
• Due to the reported cross-reactivity with human, mouse, and rat samples, consider sequence alignment of the immunogen across species if using in non-human systems.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, including validated antibody pairs and optimized monoclonal/polyclonal antibodies like this anti-MED20 polyclonal antibody. Explore our bulk ancillary reagents and rare target sourcing solutions to support your transcription and gene regulation studies throughout product development.