Anti-MED18 Rabbit Polyclonal Antibody for WB, ELISA - Q9BUE0

Core Product Specifications and Parameters

Biological Background: MED18 Function and Localization

• MED18 (Mediator complex subunit 18, also known as p28b or SRB5) is a component of the Mediator complex, a large multi-subunit coactivator required for transcription of nearly all RNA polymerase II-dependent genes.
• The Mediator complex acts as a molecular bridge, conveying regulatory signals from gene-specific transcription factors to the basal RNA polymerase II transcription machinery, and is recruited to promoters via direct interactions with regulatory proteins.
• MED18 serves as a scaffold within the Mediator to facilitate assembly of the preinitiation complex that includes RNA polymerase II and general transcription factors.
• MED18 is localized to the nucleus, consistent with its role in transcription regulation.
• Post-translational modifications include phosphorylation, which may regulate its activity or interactions.
• The Mediator complex, including MED18, is evolutionarily conserved and essential for activator-dependent transcription, as highlighted by its inclusion in structural studies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human MED18 sequence (amino acids 1-208), which may help in detecting both native and overexpressed MED18 in WB applications.
• For WB, consider using nuclear extracts or whole-cell lysates from human or mouse cell lines, as MED18 is a nuclear protein.
• ELISA may be used for quantifying MED18 levels; however, appropriate controls and validation are recommended.
• Since MED18 is part of a large complex, mild lysis conditions may be required to preserve interactions for co-immunoprecipitation experiments.
• The observed molecular weight in WB is approximately 24 kDa, which aligns with the calculated molecular weight; however, post-translational modifications might cause slight shifts.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement, offering validated antibody pairs, optimized polyclonal antibodies, and ancillary reagents for transcription research. For MED18 and other Mediator complex targets, we provide one-stop access to high-quality antibodies and bulk materials, supporting your development from concept to clinic.