Anti-MED17 Polyclonal Antibody for WB, ELISA - Q9NVC6

Core Product Specifications and Parameters

Biological Background: MED17 Function and Localization

• MED17, also known as ARC77, CRSP6, TRAP80, DRIP80, and SRB4, is a core subunit of the Mediator complex, a large multi-protein coactivator essential for regulated transcription by RNA polymerase II.
• The Mediator complex acts as a molecular bridge, conveying regulatory signals from gene-specific transcription factors to the basal transcription machinery.
• MED17 helps scaffold the assembly of the transcription preinitiation complex, facilitating RNA polymerase II recruitment to gene promoters.
• MED17 primarily localizes to the nucleus, consistent with its role in transcription.
• It is ubiquitously expressed across human tissues, reflecting its fundamental role in gene expression.
• The protein is subject to alternative splicing, and genetic variants in MED17 are associated with disease phenotypes.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 250–460 of human MED17; this region may be conserved, potentially explaining cross-reactivity with mouse and rat.
• For Western blot, a band around 73 kDa is expected; validate using positive control lysates from human, mouse, or rat cells.
• In ELISA, consider using recombinant MED17 protein or cell extracts; titration of antibody is recommended.
• Always include appropriate negative controls to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw material solutions, including validated antibodies, antigens, and ancillary reagents for diagnostic development. Our portfolio includes Mediator complex-related products like anti-MED17 antibodies, supporting research into transcriptional regulation and disease mechanisms. With expert sourcing and supply chain management, CamelBio ensures reliable access to both catalog and custom reagents for manufacturers and laboratories.