Anti-MED15 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q96RN5

Core Product Specifications and Parameters

Biological Background: MED15 Function and Localization

• MED15 (Mediator of RNA polymerase II transcription subunit 15), also known as ARC105, PCQAP, TIG1, is a component of the Mediator complex.
• The Mediator complex acts as a transcriptional coactivator for nearly all RNA polymerase II-dependent genes, bridging regulatory proteins and the basal transcription machinery.
• MED15 is recruited to promoters via direct interactions with gene-specific regulatory proteins, facilitating the assembly of the preinitiation complex with RNA polymerase II and general transcription factors.
• It positively regulates the Nodal signaling pathway and is required for cholesterol-dependent gene regulation.
• Subcellular localization includes both the nucleus and cytoplasm, consistent with its role in transcriptional regulation and potential shuttling.
• MED15 is expressed in a wide range of tissues; highest expression is observed in placenta and peripheral blood, with lower levels in pancreas and kidney.
• Post-translational modifications include phosphorylation, methylation, and ubiquitin-like conjugation, as indicated by UniProt keywords.
• The MED15 gene is associated with triplet repeat expansion and alternative splicing, yielding multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminus (aa 1–100) of human MED15. This region may recognize all major isoforms; however, isoform-specific expression should be verified if needed.
• For Western blotting (WB), a band of approximately 87 kDa is expected. Consider testing a range of antibody dilutions and optimizing blocking conditions for your sample type.
• In immunofluorescence/immunocytochemistry (IF/ICC), observe both nuclear and cytoplasmic staining. Co-staining with a nuclear marker can help confirm localization.
• For ELISA applications, this polyclonal antibody can be used as a capture or detection reagent; validate with recombinant MED15 or cell lysates.
• Cross-reactivity is reported for human, mouse, and rat. When using mouse or rat samples, confirm sequence homology to ensure comparable detection sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement for diagnostic developers and researchers. This Anti-MED15 rabbit polyclonal antibody supports investigations into Mediator complex biology and transcription regulation. Beyond standard catalog antibodies, CamelBio can source validated antibody pairs, bulk monoclonals/polyclonals, ancillary reagents, and rare-target materials to accelerate your assay development from concept to clinic.