Anti-MECR Polyclonal Antibody for IHC-P, ELISA - Q9BV79

Core Product Specifications and Parameters

Biological Background: MECR Function and Localization

• MECR (NRBF1/ETR1) functions as a mitochondrial enoyl-[acyl-carrier-protein] reductase, catalyzing the NADPH-dependent reduction of trans-2-enoyl thioesters in fatty acid synthesis type II. It accepts both ACP and CoA thioester substrates in vitro, with a preference for medium-chain fatty acids. Related references: PMID:12654921 PMID:18479707 PMID:27817865
• The enzyme may supply the octanoyl chain for lipoic acid biosynthesis, thereby regulating protein lipoylation and mitochondrial respiratory activity, notably in Purkinje cells.
• MECR is involved in iron homeostasis; it affects Fe-S cluster assembly and ceramide metabolism, and is required for maintaining proper mitochondrial morphology and bioenergetic functions. Related references: PMID:37653044
• It is localized to mitochondria, cytoplasm, and nucleus.
• Highly expressed in skeletal and heart muscle; lower expression in placenta, liver, kidney, pancreas; weak or absent in lung.
• The protein undergoes acetylation and alternative splicing, and is classified under keywords including Disease variant and Dystonia, suggesting relevance to movement disorders.
• Required for neuronal maintenance, particularly in the central nervous system.

Experimental Guidance and Technical Tips

• The immunogen (aa 54-373 of human MECR) covers a large region, likely generating polyclonal antibodies recognizing multiple epitopes. For IHC-P, consider antigen retrieval protocols and tissue-specific fixation conditions. For ELISA, validate antibody pairing with a suitable detection system.
• Cross-reactivity is reported for human; reactivity with other species should be verified in the relevant sample system.
• The predicted molecular weight is 40 kDa (UniProt: 40462 Da); confirm by western blot if used.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials, including validated antibodies for metabolic and neurological disease research. This MECR polyclonal antibody supports assays in mitochondrial biology, and our team can assist with sourcing related reagents or custom antibody development for your diagnostic needs.